Evolution of European bat lyssaviruses
Evolution of European bat lyssaviruses
复制标题
DOI：
10.1099/0022-1317-78-9-2319
复制
发表时间：
1997-09-01
影响因子：
3.8
通讯作者：
Bourhy, H
中科院分区：
文献类型：
--
作者：
Amengual, B;Whitby, JE;Bourhy, H
47个欧洲蝙蝠狂犬病毒（EEL）和两个非洲食虫蝙蝠狂犬病毒（Duvenhage病毒）被选中进行比较，以它们的进化关系。研究基于对编码核蛋白氨基末端的400个核苷酸的PCR扩增产物的直接测序。系统发育关系进行了分析后，使用最大简约法和相邻连接的方法进行自助检索。对核苷酸和氨基酸序列的分析将这些病毒分为三个独立的簇，即基因型4（Duvenhage）、基因型5（EBL 1）和基因型6（EBL 2）。进化分析的核蛋白基因的EBL 1和EBL 2表明低的内在异质性主要是由于同义替换。此外，EBL 1和EBL 2都进化成至少两个遗传上可区分的谱系（a和B），地理漂移。我们可以推测，后来的血统EBL 1a和EBL 1b被引入部分北方欧洲从两个不同的地理方向; EBL 1b可能是最近引进的，是从北非。EBL 1和鼠耳蝠、鼠耳蝠是EBL 1的主要宿主。daughonii的EBL 2的水库。
Forty-seven European bat lyssaviruses (EEL) and two African insectivorous bat lyssaviruses (Duvenhage viruses) were selected for a comparison to be made of their evolutionary relationships. Studies were based on direct sequencing of the PCR-amplified products of the 400 nucleotides coding for the amino terminus of the nucleoprotein. Phylogenetic relationships were analysed after bootstrap resampling using the maximum parsimony and the neighbour-joining methods. Analyses of both the nucleotide and amino acid sequences placed these viruses in three separate clusters, namely genotype 4 (Duvenhage), genotype 5 (EBL1) and genotype 6 (EBL2). Evolutionary analysis of the nucleoprotein gene of EBL1 and EBL2 indicated low intrinsic heterogeneity mainly due to synonymous substitutions. In addition, both EBL1 and EBL2 evolved into at least two genetically distinguishable lineages (a and b) following geographical drifting. We can speculate that subsequently the lineages EBL1a and EBL1b were introduced into parts of northern Europe from two different geographical directions; EBL1b was probably introduced most recently and was from North Africa. Eptesicus serotinus appears to be the principal reservoir for EBL1 and Myotis dasycneme and M. daubentonii the reservoirs for EBL2.