Identification, localization, and functional implications of an abundant nematode annexin.
Identification, localization, and functional implications of an abundant nematode annexin.
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DOI：
10.1083/jcb.132.6.1079
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发表时间：
1996-03
影响因子：
7.8
通讯作者：
Redick, J
中科院分区：
文献类型：
--
作者：
Creutz, CE;Snyder, SL;Daigle, SN;Redick, J
用C.检查线虫中膜联蛋白类的钙依赖性磷脂结合蛋白的存在。从线虫培养物的可溶性提取物中分离出在SDS-聚丙烯酰胺凝胶上的表观质量为32 kD的单一蛋白质，该蛋白质能够以钙依赖性方式与磷脂结合。在通过肽测序证实该蛋白为膜联蛋白后，制备该蛋白的抗血清并用于从噬菌体λ gt 11中的表达文库中分离相应的cDNA。所编码的蛋白质，在本文中称为nex-1膜联蛋白，具有35 kD的质量，并且在序列上与10种已知的哺乳动物膜联蛋白具有36-42%的同一性。在对应于钙结合位点的序列部分中发现了几个独特的修饰。线虫膜联蛋白的NH 2-末端结构域中可能的磷酸化位点对应于哺乳动物膜联蛋白的磷酸化位点。该膜联蛋白（nex-1）的基因被物理定位到染色体III的dpy-17遗传标记附近。在线虫基因组测序计划（Sulston，J.，Z. Du，K.托马斯河威尔逊湖，澳-地希利尔河Staden，N. Halloran，P.绿色，J. Thierry-Mieg，L. Qiu等人，1992年。自然（伦敦）。356：37-41）。NEX-1膜联蛋白定位在线虫的免疫荧光和电子显微镜使用免疫金标记。该蛋白与咽部分泌腺细胞的膜系统、咽部研磨器中角质层形成的位点、卵母细胞中的卵黄颗粒、子宫壁和外阴以及受精囊瓣中的膜系统相关。膜联蛋白与受精囊瓣膜的存在表明，当卵子通过受精囊瓣时，膜联蛋白在这些膜的折叠和展开中具有新的功能。本地化也表明膜联蛋白的作用对应于那些建议在哺乳动物系统中的膜运输，胶原蛋白沉积，和细胞外基质的形成。
Cultures of the nematode C. elegans were examined for the presence of calcium-dependent, phospholipid-binding proteins of the annexin class. A single protein of apparent mass on SDS-polyacrylamide gels of 32 kD was isolated from soluble extracts of nematode cultures on the basis of its ability to bind to phospholipids in a calcium-dependent manner. After verification of the protein as an annexin by peptide sequencing, an antiserum to the protein was prepared and used to isolate a corresponding cDNA from an expression library in phage lambda gt11. The encoded protein, herein referred to as the nex-1 annexin, has a mass of 35 kD and is 36-42% identical in sequence to 10 known mammalian annexins. Several unique modifications were found in the portions of the sequence corresponding to calcium-binding sites. Possible phosphorylation sites in the NH2-terminal domain of the nematode annexin correspond to those of mammalian annexins. The gene for this annexin (nex-1) was physically mapped to chromosome III in the vicinity of the dpy-17 genetic marker. Two other annexin genes (nex-2 and nex-3) were also identified in chromosome III sequences reported by the nematode genomic sequencing project (Sulston, J., Z. Du, K. Thomas, R. Wilson, L. Hillier, R. Staden, N. Halloran, P. Green, J. Thierry-Mieg, L. Qiu, et al. 1992. Nature (Lond.). 356:37-41). The nex-1 annexin was localized in the nematode by immunofluorescence and by electron microscopy using immunogold labeling. The protein is associated with membrane systems of the secretory gland cells of the pharynx, with sites of cuticle formation in the grinder in the pharynx, with yolk granules in oocytes, with the uterine wall and vulva, and with membrane systems in the spermathecal valve. The presence of the annexin in association with the membranes of the spermathecal valve suggests a novel function of the protein in the folding and unfolding of these membranes as eggs pass through the valve. The localizations also indicate roles for the annexin corresponding to those proposed in mammalian systems in membrane trafficking, collagen deposition, and extracellular matrix formation.
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影响因子：
2.9
作者：
FUNAKOSHI, T;HENDRICKSON, LE;FUJIKAWA, K
通讯作者：
FUJIKAWA, K
影响因子：
64.5
作者：
NONET, ML;GRUNDAHL, K;RAND, JB
通讯作者：
RAND, JB
影响因子：
3.2
作者：
ITO, H;FUKUDA, Y;KIMURA, A
通讯作者：
KIMURA, A
DOI：
10.1073/pnas.86.10.3798
发表时间：
1989-05-01
影响因子：
11.1
作者：
BURNS, AL;MAGENDZO, K;POLLARD, HB
通讯作者：
POLLARD, HB
DOI：
10.1111/j.1432-1033.1991.tb16076.x
发表时间：
1991-06-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BARTON, GJ;NEWMAN, RH;CRUMPTON, MJ
通讯作者：
CRUMPTON, MJ