Lignin Biosynthesis Gene Expression Is Associated with Age-related Resistance of Winter Squash to Phytophthora capsici
Lignin Biosynthesis Gene Expression Is Associated with Age-related Resistance of Winter Squash to Phytophthora capsici
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DOI：
10.21273/jashs05317-23
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发表时间：
2023-09
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影响因子：
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通讯作者：
S. Alzohairy;Bethany M. Moore;Raymond Hammerschmidt;Shin-Han Shiu;M. Hausbeck
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作者：
S. Alzohairy;Bethany M. Moore;Raymond Hammerschmidt;Shin-Han Shiu;M. Hausbeck
卵菌纲植物病原体辣椒疫霉（Phytophthora capsici）引起冬南瓜（Cucurbita moschata）的根、冠和果实腐烂并限制产量。一些C。moschata品种发展年龄相关的阻力（ARR），其中水果发展抗辣椒疫霉授粉后14至21天（DPP），因为加厚外果皮;然而，受伤否定ARR。揭示了两种C. Moschata栽培品种，Chieftain和Dickenson Field，使用RNA测序，分别在14和21 DPP时显示ARR。使用在7、10、14和21 DPP下来自“Chieftain”和“Dickenson Field”果实的RNA样品进行测序。差异表达和随后的基因集富集分析揭示了与细胞壁结构生物合成、细胞壁修饰/组织、转录调控和代谢过程相关的功能类别中上调基因的过度表达。一个途径富集分析检测上调基因角质，木栓素单体，苯丙素类生物合成途径。对这些途径中的基因表达谱的进一步分析揭示了抗性果皮中单乙醇生物合成和木质素聚合中的基因的上调。我们的研究结果表明，在基因表达的转变，对物理加强细胞壁与ARR辣椒疫霉。这些发现为开发抗辣椒疫霉的南瓜品种和改善南瓜种果实腐烂病的管理提供了候选基因。
The Oomycete plant pathogen, Phytophthora capsici, causes root, crown, and fruit rot of winter squash (Cucurbita moschata) and limits production. Some C. moschata cultivars develop age-related resistance (ARR), whereby fruit develop resistance to P. capsici 14 to 21 days postpollination (DPP) because of thickened exocarp; however, wounding negates ARR. We uncovered the genetic mechanisms of ARR of two C. moschata cultivars, Chieftain and Dickenson Field, that exhibit ARR at 14 and 21 DPP, respectively, using RNA sequencing. The sequencing was conducted using RNA samples from ‘Chieftain’ and ‘Dickenson Field’ fruit at 7, 10, 14, and 21 DPP. A differential expression and subsequent gene set enrichment analysis revealed an overrepresentation of upregulated genes in functional categories relevant to cell wall structure biosynthesis, cell wall modification/organization, transcription regulation, and metabolic processes. A pathway enrichment analysis detected upregulated genes in cutin, suberin monomer, and phenylpropanoid biosynthetic pathways. A further analysis of the expression profile of genes in those pathways revealed upregulation of genes in monolignol biosynthesis and lignin polymerization in the resistant fruit peel. Our findings suggest a shift in gene expression toward the physical strengthening of the cell wall associated with ARR to P. capsici. These findings provide candidate genes for developing Cucurbita cultivars with resistance to P. capsici and improve fruit rot management in Cucurbita species.