TIMING OF TRANSCRIPTION AND PROTEIN-SYNTHESIS UNDERLYING MORPHOGENESIS IN PREIMPLANTATION MOUSE EMBRYOS
TIMING OF TRANSCRIPTION AND PROTEIN-SYNTHESIS UNDERLYING MORPHOGENESIS IN PREIMPLANTATION MOUSE EMBRYOS
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DOI：
10.1016/0012-1606(85)90397-5
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
MCLACHLIN, JR
中科院分区：
文献类型：
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作者：
KIDDER, GM;MCLACHLIN, JR
在小鼠着床前发育过程中，胚胎经历一系列形态发生事件：压实、形成囊胚腔的液体积聚（空化）和从透明带中逃脱（孵化）。我们使用了抑制剂。-amanitin和环己亚胺来研究这些形态发生阶段的转录和翻译事件的时间。每隔一定的时间，将一组胚胎从一个公共池转移到含有一种或另一种抑制剂的培养基中，然后在随后的24小时或更长时间内对它们达到特定形态发生终点的能力进行评分。通过比较对照群体到达终点的时间和为了防止它们到达终点而必须将胚胎转移到抑制剂中的时间，我们可以提前确定每一个甚至必要的转录或蛋白质合成完成的时间。我们的研究结果表明，尽管必要的转录提前完成，至少在早期的四细胞阶段完成，但压实（以及分裂到八细胞阶段）是胚胎而不是母体编程的事件。另一方面，流体积聚背后的转录和翻译事件似乎在这一过程开始后的几个小时内完成。对于孵化来说，在完成必要转录事件的表面时间和过程本身之间又有很长的延迟，蛋白质合成只提前几个小时完成。我们的研究结果提出了转录后调控机制在早期小鼠胚胎形态发生事件的时间中发挥重要作用的可能性。
During preimplantation development of the mouse, embryos pass through a series of morphogenetic events: compaction, fluid accumulation to form the blastocoele (cavitation), and escape from the zona pellucida (hatching). We have used the inhibitors .alpha.-amanitin and cycloheximide to investigate the timing of transcriptional and translational events underlying these morphogenetic stages. Groups of embryos were transferred from a common pool into medium containing one or the other inhibitor at regular time intervals, and then were scored over the ensuing 24 or more hours for their ability to reach a particular morphogenetic end point. By comparing the time when the control population reached an end point with the time at which embryos had to be transferred into the inhibitor in order to prevent them from reaching that end point, we could determine when in advance of each even the necessary transcription or protein synthesis has been completed. Our results suggest that compaction (as well as cleavage to the eight-cell stage) is an embryonically, rather than maternally, programmed event, although the necesary transcription is completed well in advance, at least by the early four-cell stage. The transcriptional and translational events underlying fluid accumulation, on the other hand, appear to be completed within a few hours of the start of this process. For hatching, there is once again a long delay between the apparent time of completion of the necessary transcriptional events and the process itself, with protein synthesis being completed just a few hours in advance. Our results raise the possibility that posttranscriptional regulatory mechanisms play an important role in the timing of morphogenetic events in early mouse embryos.