Controllable release of dopamine from simulated enzyme-containing biomembrane by biased potential
Controllable release of dopamine from simulated enzyme-containing biomembrane by biased potential
复制标题
通过偏置电位从模拟含酶生物膜中可控释放多巴胺
DOI：
10.1016/j.aca.2020.05.040
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发表时间：
2020
影响因子：
6.2
通讯作者：
Huibo Shao
中科院分区：
文献类型：
--
作者：
Jingchao Chen;Ximing Huang;Chunxia Yan;Haixia Guo;Zhiming Gao;Huibo Shao
多巴胺（DA）的可控释放在生理代谢过程中具有重要意义，值得研究。构建了基于氢键网络的β-环糊精/11-巯基十一酸自组装膜（β-CD/MUA SAM），作为模拟含酶生物膜。DA与β-CD通过分子间氢键作用形成包合物，即DA@β-CD/MUA SAM。DA从DA@β-CD/MUA SAM上的脱附过程从分子水平揭示了DA从含酶生物膜上的释放行为。在DA@β-CD/MUA SAM处施加正偏压作为外部刺激，降低SAM的负电荷密度。SAM的负电荷密度降低导致DA与β-CD之间的氢键减弱，从而导致DA释放。用扫描电化学显微镜观察，正偏压不仅对DA的释放具有特异性诱导作用，而且能够真实的实时定量监测DA的释放。这些结果表明，正偏压作为外刺激有利于DA的可控释放，提示偏压电位在可控调节领域的可能应用。
Controllable release of dopamine (DA) is worth studying for its significant in physiological metabolic process. β-cyclodextrin/11-mercaptoundecanoic acid self-assembled monolayer (β-CD/MUA SAM) based on hydrogen bonds network was constructed as simulated enzyme-containing biomembrane. DA interacted with β-CD based on intermolecular hydrogen bond and formed inclusion complexes in SAM, namely DA@β-CD/MUA SAM. The desorption process of DA from DA@β-CD/MUA SAM revealed the release behavior of DA from enzyme-containing biomembrane at molecular level. Positive biased potential as external stimulus was applied at DA@β-CD/MUA SAM, reducing the negative charge density of SAM. The decrease of negative charge density of SAM resulted in the weakening of hydrogen bond between DA and β-CD, which in turn caused DA to be released. Using scanning electrochemical microscopy, positive biased potential not only had specificity induction for the release of DA, but also was able to monitor the release quantitatively in real time. These results showed that positive biased potential as external stimulus was favorable for the controllable release of DA, suggesting the possible application of biased potential in controllable regulation field.