Glucose Oxidase Directly Immobilized onto Highly Porous Gold Electrodes for Sensing and Fuel Cell applications
Glucose Oxidase Directly Immobilized onto Highly Porous Gold Electrodes for Sensing and Fuel Cell applications
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DOI：
10.1016/j.electacta.2014.06.074
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发表时间：
2014-08-20
影响因子：
6.6
通讯作者：
Di Lorenzo, Mirella
中科院分区：
文献类型：
--
作者：
du Toit, Hendrik;Di Lorenzo, Mirella
氧化还原-酶电极在生物传感器和酶生物燃料电池中的成功应用一直是人们研究的热点，而蛋白质-电极界面的有效电子传递对电极的高灵敏度和高能量转换效率起着关键作用。这是很难实现的情况下，葡萄糖氧化酶，由于事实上，这种酶的氧化还原中心被埋在结构内，远离任何可行的电极binding sites.This study reports，一个简单而快速的方法直接固定葡萄糖氧化酶到高度多孔的金电极。当将所得电极作为葡萄糖传感器进行测试时，观察到Michaelis-Menten动力学趋势，检测限为25 μ M。根据生物电极的表面积计算，生物电极的灵敏度为22.7 +/- 0.1 μ A mM（-1）cm（-2）。该葡萄糖氧化酶电极还作为葡萄糖/O-2酶生物燃料电池的阳极进行了测试，在0.2V的电势下产生6 μ W cm（-2）的峰值功率密度。(C)2014作者由爱思唯尔有限公司发布
The successful implementation of redox-enzyme electrodes in biosensors and enzymatic biofuel cells has been the subject of extensive research.For high sensitivity and high energy-conversion efficiency, the effective electron transfer at the protein-electrode interface has a key role. This is difficult to achieve in the case of glucose oxidase, due to the fact that for this enzyme the redox centre is buried inside the structure, far from any feasible electrode binding sites.This study reports, a simple and rapid methodology for the direct immobilisation of glucose oxidase into highly porous gold electrodes. When the resulting electrode was tested as glucose sensor, a Michaelis-Menten kinetic trend was observed, with a detection limit of 25 mu M. The bioelectrode sensitivity, calculated against the superficial surface area of the bioelectrode, was of 22.7 +/- 0.1 mu A mM(-1) cm(-2).This glucose oxidase electrode was also tested as an anode in a glucose/O-2 enzymatic biofuel cell, leading to a peak power density of 6 mu W cm(-2) at a potential of 0.2V. (C) 2014 The Authors. Published by Elsevier Ltd.