UCP1 expression in the mouse adrenal gland is not upregulated by thermogenic conditions
UCP1 expression in the mouse adrenal gland is not upregulated by thermogenic conditions
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DOI：
10.1016/j.bbrc.2021.06.013
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发表时间：
2021-06-12
影响因子：
3.1
通讯作者：
Ohuchi, Hideyo
中科院分区：
文献类型：
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作者：
Fujita, Hirofumi;Habuta, Munenori;Ohuchi, Hideyo
解偶联蛋白1(UCP1)基因在具有产热功能的棕色脂肪组织(BAT)中高表达。已有研究表明，UCP1mRNA定位于小鼠肾上腺，但其意义尚不清楚。为了探索UCP1在肾上腺中的表达是如何调节的，我们利用CRISPR-Cas9系统将GFP基因插入到UCP1基因座中，建立了一个报告敲除小鼠。首先，通过Western印迹分析，我们证实了UCP1驱动的GFP蛋白在4℃敲入小鼠肩关节间蝙蝠中的表达。免疫组织化学显示，敲入小鼠的肾上腺中检测到了GFP蛋白。无论冷暴露与否，在报告小鼠的肾上腺髓质中观察到比在皮质中更强的GFP表达。使用抗UCP1抗体的免疫组织化学以及Western印迹分析证实了UCP1蛋白在野生型肾上腺髓质中的表达。这些结果表明，小鼠肾上腺是一个表达UCP1蛋白的新器官，其表达不受冷暴露的影响。(C)2021 Elsevier Inc.保留所有权利。
The uncoupling protein 1 (UCP1) gene is known to be highly expressed in brown adipose tissue (BAT) that functions in thermogenesis. It has been shown that UCP1 mRNA is localized to the mouse adrenal gland, but its significance remains elusive. To explore how UCP1 expression in the adrenal gland is regulated, we generated a reporter knock-in mouse in which the GFP gene was inserted into the UCP1 locus using CRISPR-Cas9 system. Firstly, we confirmed by Western blot analysis UCP1-driven GFP protein expression in interscapular BAT of the knock-in mice kept at 4 degrees C. Immunohistochemistry showed that GFP protein was detected in the adrenal gland of the knock-in mice. More intense GFP expression was observed in the adrenal medulla than in the cortex of the reporter mice irrespectively of cold exposure. Immunohistochemistry using anti-UCP1 antibody, as well as Western blot analysis verified UCP1 protein expression in the wild-type adrenal medulla. These results suggest that the mouse adrenal gland is a novel organ expressing UCP1 protein and its expression is not upregulated by cold exposure. (C) 2021 Elsevier Inc. All rights reserved.