Insecticidal proteins from Bacillus thuringiensis protect corn from corn rootworms

Insecticidal proteins from Bacillus thuringiensis protect corn from corn rootworms
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DOI:
10.1038/90282
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发表时间:
2001-07-01
影响因子:
46.9
通讯作者:
Duck, N
Duck, N
中科院分区:
工程技术1区
文献类型:
--
作者:
Moellenbeck, DJ;Peters, ML;Duck, N

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玉米的田间试验共表达两种新的δ-内毒素从苏云金芽孢杆菌(Bt)已证明保护根损害西方玉米根虫(玉米根萤叶甲)。保护水平超过了化学杀虫剂。在细菌中,这些蛋白质在生长周期的孢子形成阶段形成晶体,由单个操纵子编码,分子量为14 kDa和44 kDa。以表达蛋白质的玉米根喂养的玉米根虫幼虫在中肠上皮中表现出组织病理学症状。
Field tests of corn co-expressing two new delta-endotoxins from Bacillus thuringiensis (Bt) have demonstrated protection from root damage by western corn rootworm (Diabrotica virgifera virgifera LeConte). The level of protection exceeds that provided by chemical insecticides. In the bacterium, these proteins form crystals during the sporulation phase of the growth cycle, are encoded by a single operon, and have molecular masses of 14 kDa and 44 kDa. Corn rootworm larvae fed on corn roots expressing the proteins showed histopathological symptoms in the midgut epithelium.