Enhanced effect of photodynamic therapy in ovarian cancer using a nanoparticle drug delivery system
Enhanced effect of photodynamic therapy in ovarian cancer using a nanoparticle drug delivery system
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DOI：
10.3892/ijo.2015.3079
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发表时间：
2015-09-01
影响因子：
5.2
通讯作者：
Kong, Beihua
中科院分区：
文献类型：
--
作者：
Li, Zhao;Sun, Liping;Kong, Beihua
纳米粒是一种具有肿瘤靶向性和药物控释功能的新型药物载体。本研究制备竹红菌素B（HB）聚氰基丙烯酸正丁酯纳米粒（PBCA-NP），以提高光动力学疗法（PDT）对卵巢癌的疗效。采用Fischer 344大鼠和HB包埋PBCA-NP（HB-PBCA-NP）成功建立了卵巢癌腹水模型。通过与HB二甲基亚砜（HB-DMSO）的比较和在腹腔内给药后不同时间点的测试，评价HB-PBCA-NP系统的药效学特征和生物分布。然后将基于HB-PBCA-NP的PDT联合细胞减灭术施用于荷瘤动物。进行Kaplan-Meier生存分析以评估纳米颗粒系统的治疗效果。血清HB浓度在纳米颗粒系统中给药后4 h达到峰值，HB-DMSO给药后1 h达到峰值。肿瘤组织的峰值暴露时间也延长（4小时对2小时），PBCA-NP保持存在的时间比HB-DMSO长得多。虽然PDT联合手术较单纯手术明显延长生存期（84天，P
Nanoparticles are promising novel drug delivery carriers that allow tumor targeting and controlled drug release. In the present study, we prepared poly butyl-cyanoacrylate nanoparticles (PBCA-NP) entrapped with hypocrellin B (HB) to improve the effect of photodynamic therapy (PDT) in ovarian cancer. An ovarian cancer ascites model using Fischer 344 rats and PBCA-NP entrapped with HB (HB-PBCA-NP) were formed successfully. The pharmacodynamic characteristics and biodistribution of the HB-PBCA-NP system were evaluated by comparison with HB dimethyl sulfoxide (HB-DMSO) and testing at various time-points following intraperitoneal drug administration. HB-PBCA-NP-based PDT combined with cytoreductive surgery was then administrated to the tumor-bearing animals. Kaplan-Meier survival analysis was performed to assess the therapeutic effect of the nanoparticle system. The serum HB concentration peaked 4 h after drug administration in the nanoparticle system, and 1 h with HB-DMSO. The peak exposure time of tumor tissues was also extended (4 vs. 2 h), and PBCA-NP remained present for much longer than HB-DMSO. Although PDT combined with surgery prolonged the survival time significantly compared with surgery alone (84 days, P