Induction of myelogenous leukemia cells with histone deacetylase inhibitors through down-regulating the Daxx protein expression
Induction of myelogenous leukemia cells with histone deacetylase inhibitors through down-regulating the Daxx protein expression
复制标题
组蛋白脱乙酰酶抑制剂通过下调 Daxx 蛋白表达诱导骨髓性白血病细胞
DOI：
10.1007/s11596-009-0504-7
复制
发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
Deng, J.
中科院分区：
文献类型：
--
作者：
Wu, X.;Liu, W.;Li, C.;Zhou, J.;Tian, Y.;Deng, J.
研究了组蛋白去乙酰化酶（HDAC）抑制剂丁酸钠（NaB）和曲古霉素A （TSA）对人白血病细胞体外凋亡的影响及其分子机制。实验分为5组：对照组、NaB组、TSA组、NaB+Z-VAD-FMK组和TSA+Z-VAD-FMK组。形态学分析和流式细胞术检测细胞凋亡率。Western blot检测Daxx、Bcl-2、Bcl-xl蛋白的表达。NaB和TSA均能诱导HL-60和K562细胞凋亡，Z-VAD-FMK能显著降低HDAC抑制剂诱导的细胞凋亡。HDAC抑制剂可下调Daxx蛋白的表达，但对Bcl-2和Bcl-xl蛋白的表达无显著影响。结果表明，NaB和TSA通过下调体外Daxx蛋白的表达诱导人白血病细胞明显的caspase依赖性凋亡。
The effects of two different histone deacetylase (HDAC) inhibitors, sodium butyrate (NaB) and trichostatin A (TSA),on apoptosis of human leukemic cellsin vitroand the molecular mechanisms were investigated. The experiments were divided up 5 groups: control group, NaB group, TSA group, NaB+Z-VAD-FMK group and TSA+Z-VAD-FMK group. The apoptosis rate was determined by morphological analysis and flow cytomytry. The expression of Daxx, Bcl-2, and Bcl-xl proteins was detected by Western blot. NaB and TSA could induce the apoptosis of HL-60 and K562 cells, and Z-VAD-FMK caused a marked decrease in apoptosis induced by HDAC inhibitors. HDAC inhibitors could down-regulate the expression of Daxx protein, but had no significant influence on the expression of Bcl-2 and Bcl-xl proteins. The results suggested that NaB and TSA induce distinct caspase-dependent apoptosis of human leukemic cells through down-regulating the expression of Daxx proteinin vitro.
登录
查看更多内容
影响因子：
4.8
作者：
Garcia-Calvo, M;Peterson, EP;Thornberry, NA
通讯作者：
Thornberry, NA
影响因子：
2.3
作者：
Kim, Byung-Su;Bae, Eunkyung;Yoon, Sung-Soo
通讯作者：
Yoon, Sung-Soo
影响因子：
2.7
作者：
Nishioka, Chie;Ikezoe, Takayuki;Yokoyama, Akihito
通讯作者：
Yokoyama, Akihito
影响因子：
2
作者：
G. Bartolini;M. Orlandi;A. Papi;K. Ammar;R. Tonelli;M. Franzoni;A. Pession;P. Rocchi;A. Ferreri-A.-Fer
通讯作者：
G. Bartolini;M. Orlandi;A. Papi;K. Ammar;R. Tonelli;M. Franzoni;A. Pession;P. Rocchi;A. Ferreri-A.-Fer
影响因子：
5.5
作者：
Schmidt, Kerstin;Seeger, Karl;Moldenhauer, Anja
通讯作者：
Moldenhauer, Anja