Vitreous fluorophotometry: identification of sources of variability.
Vitreous fluorophotometry: identification of sources of variability.
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玻璃体荧光光度法：识别变异性来源。
DOI：
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发表时间：
1981
影响因子：
4.4
通讯作者：
R. Anderson
中科院分区：
文献类型：
--
作者：
T. Prager;D. Wilson;G. Avery;J. Merritt;C. Garcia;G. Hopen;R. Anderson
裂隙灯玻璃体荧光光度法（VF）已被几个研究者用来记录早期破坏的血眼屏障。在注射荧光素后，据报道，与对照组相比，糖尿病患者玻璃体液中的荧光素水平升高，即使当通过血管造影术或检眼镜检查没有可辨别的视网膜病理状况时。我们已经研究了正常人的VF技术，并确定了几个潜在的错误来源，在玻璃体荧光素水平的测定。脉络膜循环中的黄绿素显著影响在视网膜表面和后玻璃体中获取的VF读数。这种效应受眼部色素沉着的调节，深色眼睛正常人的重度色素沉着比蓝眼睛受试者的轻度色素沉着更有效地筛选出脉络膜荧光。脉络膜荧光素的影响向玻璃体中部延伸，并且影响范围与激发光的狭缝宽度直接相关。在窄缝宽度的影响是减弱，但永远不能完全消除。血液荧光素水平随时间降低，并且由于脉络膜荧光素有助于VF值，因此注射后获得VF阅读的时间是关键参数。
Slit-lamp vitreous fluorophotometry (VF) has been used by several investigators to document the early breakdown of the blood-ocular barrier. After an injection of fluorescein, levels of fluorescein in the vitreous humor are reported to be elevated in diabetic patients compared with controls even when there is no retinal pathologic condition discernible by angiography or ophthalmoscopy. We have examined the technique of VF in normal persons and have identified several potential sources of error in the determination of vitreous fluorescein levels. Fluorescein in the choroidal circulation significantly influences VF readings taken at the retinal surface and in the posterior vitreous. This effect is modulated by ocular pigmentation, with heavy pigmentation of dark-eyed normals more effectively screening out choroidal fluorescence than the light pigmentation of blue-eyed subjects. Influence of choroidal fluorescein extends toward the midvitreous, and the range of influence is directly related to the slit width of the exciting light. At narrow slit widths the effect is diminished but can never be completely eliminated. Blood fluorescein levels decrease with time, and since choroidal fluorescein contributes to VF values, the time after injection that VF reading are taken is a critical parameter.
实验性糖尿病血视网膜屏障的临床病理学研究。
DOI：
10.1001/archopht.1980.01020040884020
发表时间：
1980
期刊：
Archives of ophthalmology (Chicago, Ill. : 1960)
影响因子：
--
作者：
Tso,MO;Cunha-Vaz,JG;Shih,CY;Jones,CW
通讯作者：
Jones,CW