Chemically amplified 19F-1H nuclear Overhauser effects
Chemically amplified 19F-1H nuclear Overhauser effects
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DOI：
10.1016/j.jmr.2004.01.011
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发表时间：
2004-05-01
影响因子：
2.2
通讯作者：
Hore, PJ
中科院分区：
文献类型：
--
作者：
Kuprov, I;Hore, PJ
化学诱导动态核极化（CIDNP）被探索为异核欧沃豪塞效应实验中核超极化的来源。通过自由基对中间体进行的光化学反应用于将 3-氟酪氨酸中的 F-19 核磁化强度增强一个数量级以上，同时 F-19-H-1 交叉弛豫和互相关效应的幅度相应增加。所采用的反应是循环的，并且使样品的化学性质保持不变。讨论了增强 F-19 标记蛋白中异核 NOE 敏感性的潜力。 (C) 2004 Elsevier Inc. 保留所有权利。
Chemically induced dynamic nuclear polarisation (CIDNP) is explored as a source Of nuclear hyperpolarisation in heteronuclear Overhauser effect experiments. A photochemical reaction proceeding through a radical pair intermediate is used to enhance F-19 nuclear magnetisation in 3-fluorotyrosine by more than an order of magnitude with a corresponding increase in the amplitudes of F-19-H-1 cross-relaxation and cross-correlation effects. The reactions employed are cyclic and leave the sample chemically unchanged. The potential for enhancing the sensitivity Of heteronuclear NOEs in F-19-labelled proteins is discussed. (C) 2004 Elsevier Inc. All rights reserved.