Meeting report: The use of newborn blood spots in environmental research: Opportunities and challenges
Meeting report: The use of newborn blood spots in environmental research: Opportunities and challenges
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DOI：
10.1289/ehp.10511
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发表时间：
2007-12-01
影响因子：
10.4
通讯作者：
Olshan, Andrew F.
中科院分区：
文献类型：
--
作者：
Olshan, Andrew F.
简介：在美国，通常使用足跟棒从新生儿身上采集干血斑 (DBS)。 DBS 用于筛查先天性代谢缺陷和其他疾病。更多的州正在保留剩余的斑点并将其用于研究目的。 DBS提取DNA已得到广泛应用；然而，开发在 DBS 中测量一系列环境毒物的方法一直是实验室科学家和流行病学家的近期目标。 目的：会议的目的是检查 DBS 在测量环境暴露方面的效用。演讲者和讨论者受邀展示数据并讨论使用 DBS 测量一系列分析物的方法。 结果：本次会议于 2007 年 2 月 20 日在北卡罗来纳大学教堂山分校举行。观众包括流行病学家、化学家以及来自州公共卫生项目、疾病控制和预防中心以及美国国立卫生研究院的工作人员。会议包括关于阻燃化学品和农药、金属、高氯酸盐、传染性病原体、免疫状态标记物和蛋白质加合物测量的介绍。分析方法包括质谱、原子吸收、分子方法和微流体技术。据报告取得了重大进展，但仍然存在重大挑战。包括储存条件、样品量、污染和标准化在内的问题需要额外的系统评估。此外，DBS存储和访问策略需要协调。结论。 DBS 仍然是临床、流行病学和毒理学研究的非常有价值的资源。使用 DBS 来测量环境暴露显示出了希望，但在保证更广泛的使用之前还需要做更多的工作。
INTRODUCTION: Dried blood spots (DBS) are routinely collected from newborns in the United States using a heel stick. The DBS are screened for inborn errors of metabolism and other disorders. More states are keeping residual spots and making them available for research purposes. DNA extraction from the DBS has been widely applied; however, the development of methods to measure a range of environmental toxicants in DBS has been a more recent goal for laboratory scientists and epidemiologists.OBJECTIVE: The purpose of the meeting was to examine the utility of DBS to measure environmental exposures. Speakers and discussants were invited to present data and discuss approaches to measure a range of analytes using DBS.RESULTS: This meeting was held on 20 February 2007 at the University of North Carolina at Chapel Hill. The audience consisted of epidemiologists, chemists, and staff from state public health programs, the Centers for Disease Control and Prevention, and the National Institutes of Health. The meeting included presentations on measurement of flame-retarding chemicals and pesticides, metals, Perchlorate, infectious agents, markers of immune status, and protein adducts. Analytical methods included mass spectrometry, atomic absorption, molecular methods, and microfluidic techniques. Significant progress was reported, but important challenges remain. Concerns including storage conditions, sample volume, contamination, and normalization require additional systematic evaluation. In addition, DBS storage and access policies require coordination.CONCLUSIONS. DBS remain a highly valuable resource for clinical, epidemiologic, and toxicologic investigation. The use of DBS to measure environmental exposures shows promise but additional work is necessary before more widespread use is warranted.