Printing of protein microarrays via a capillary-free fluid jetting mechanism
Printing of protein microarrays via a capillary-free fluid jetting mechanism
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DOI：
10.1002/pmic.200401294
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发表时间：
2005-11-01
期刊：
影响因子：
3.4
通讯作者：
Ringeisen, BR
中科院分区：
文献类型：
--
作者：
Barron, JA;Young, HD;Ringeisen, BR
目前的蛋白质组学实验依赖于打印技术，如喷墨，针，或羽毛管阵列，开发用于创建cDNA微阵列。这些技术通常不能满足成功点样蛋白质以进行高通量、基于阵列的蛋白质组学分析所需的要求。生物激光打印（BioLP）是一种点样技术，不依赖于固体针，羽毛针或基于毛细管的流体。BioLP的非接触机制利用聚焦激光脉冲转移蛋白质溶液，从而消除了市售点样技术中可能遇到的孔堵塞、气泡和不必要的体积损失的可能性。BioLP的速度和点到点的再现性与其他技术相当，而每个打印液滴的最小点直径和体积分别在30 gm和500 fL时明显较小。BioLP通过流体喷射机制进行流体转移，如打印过程的高速图像所示。将BSA溶液阵列化，随后进行免疫检测，证明了在具有以下CV的阵列形式中蛋白质的可再现点样：
Current proteomics experiments rely upon printing techniques such as ink jet, pin, or quill arrayers that were developed for the creation of cDNA microarrays. These techniques often do not meet the requirements needed for successful spotting of proteins to perform high-throughput, array-based proteomic profiling. Biological laser printing (BioLP) is a spotting technology that does not rely on solid pins, quill pins, or capillary-based fluidics. The non-contact mechanism of BioLP utilizes a focused laser pulse to transfer protein solutions, thereby eliminating the potential for orifice clogging, air bubbles, and unnecessary volume loss potentially encountered in commercially available spotting technologies. The speed and spot-to-spot reproducibility of BioLP is comparable to other techniques, while the minimum spot diameter and volume per printed droplet is significantly less at 30 gm and similar to 500 fL, respectively. The transfer of fluid by BioLP occurs through a fluid jetting mechanism, as observed by high-speed images of the printing process. Arraying a solution of BSA with subsequent immunodetection demonstrates the reproducible spotting of protein in an array format with CVs of