A common-lines based method for determining orientations for N>3 particle projections simultaneously

A common-lines based method for determining orientations for N>3 particle projections simultaneously
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DOI:
10.1016/0304-3991(96)00037-x
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发表时间:
1996-07-01
期刊:
影响因子:
2.2
通讯作者:
Frank, J
Frank, J
中科院分区:
工程技术3区
文献类型:
--
作者:
Penczek, PA;Zhu, J;Frank, J

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提出了一种确定投影方向的方法。同时使用任意数量的未知三维结构的投影作为输入。该方法以公共线为基础,采用一种新的差异度量,考虑了公共线在角空间上的不均匀分布。应用70 S大肠杆菌核糖体的数据从能量过滤电子显微镜得到的描述。
A method is proposed for determining the directions of projections. An arbitrary number of projections of unknown three-dimensional structure are simultaneously used as input. The method is based on common lines and uses a new discrepancy measure accounting for the uneven distribution of common lines ir. angular space. An application to the 70S Escherichia coli ribosome data obtained from an energy-filtering electron microscope is described.