The effect of insulin in combination with selenium on blood glucose and GLUT4 expression in the cardiac muscle of streptozotocin‐induced diabetic rats
The effect of insulin in combination with selenium on blood glucose and GLUT4 expression in the cardiac muscle of streptozotocin‐induced diabetic rats
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DOI：
10.1111/j.1472-8206.2009.00715.x
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发表时间：
2009-12
影响因子：
2.9
通讯作者：
T. Xu;B. Yuan;Ya-Min Zou;W. Zang
中科院分区：
文献类型：
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作者：
T. Xu;B. Yuan;Ya-Min Zou;W. Zang
我们观察了小剂量胰岛素(1U/kg/d)和硒(180U/kg/d)联合应用对链脲佐菌素诱导的糖尿病大鼠一般生理参数和心肌葡萄糖转运体(GluT4)水平的影响。糖尿病大鼠分别给予胰岛素、硒及胰岛素和硒联合治疗4周。测定血糖和糖化血红蛋白A1c水平，免疫印迹和免疫组织化学方法检测心肌组织GLUT4水平。胰岛素联合硒能显著降低血糖和糖化血红蛋白水平，并能恢复心肌GLUT4水平的紊乱。胰岛素治疗在恢复糖尿病的改变方面只有部分有效。结论：胰岛素和硒之间存在协同作用，联合应用胰岛素和硒治疗糖尿病大鼠能有效地控制血糖，纠正糖尿病大鼠心脏GLUT4分布的改变。
We evaluated the effect of a combination of low doses of insulin (1 U/kg/day) and selenium (180 μg/kg/day) on general physiological parameters and the level of glucose transporter (GLUT4) in the cardiac muscle of streptozotocin‐induced diabetic rats. Diabetic rats were treated with insulin, selenium and a combination of insulin and selenium for 4 weeks. The levels of blood glucose and hemoglobin A1c were estimated; the level of the GLUT4 in the cardiac muscle was examined by immunoblotting and immunohistochemistry. Insulin in combination with selenium could significantly lower blood glucose and HbA1c levels and could restore disturbances in GLUT4 level in the cardiac muscle. The treatment with insulin was only partially effective in the restoration of diabetic alterations. We conclude that there was cooperation between insulin and selenium, and that the treatment of diabetic rats with combined doses of insulin and selenium was effective in the control of blood glucose and correction of altered GLUT4 distribution in diabetic rat hearts.