Evaluation of drug effects on cerebral blood flow and glucose uptake in un-anesthetized and un-stimulated rats: application of free-moving apparatus enabling to keep rats free during PET/SPECT tracer injection and uptake.
Evaluation of drug effects on cerebral blood flow and glucose uptake in un-anesthetized and un-stimulated rats: application of free-moving apparatus enabling to keep rats free during PET/SPECT tracer injection and uptake.
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DOI：
10.1097/mnm.0000000000000863
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发表时间：
2018-08
影响因子：
1.5
通讯作者：
Magata Y
中科院分区：
文献类型：
--
作者：
Sugita T;Kondo Y;Ishino S;Mori I;Horiguchi T;Ogawa M;Magata Y
本研究的目的是开发新的氟-18-氟脱氧葡萄糖（18F-FDG）-PET和99 mTc-六甲基丙烯胺肟（HMPAO）SPECT方法与自由移动装置在清醒大鼠上研究大脑活动，而没有麻醉和触觉刺激的影响。我们还评估了敏感性的实验系统的干预研究使用氟西汀作为参考药物。将导管插入股静脉并连接至自由移动的插管系统。氟西汀给药后，大鼠经静脉插管注射18F-FDG或99 mTc-HMPAO，并释放到自由活动的笼子中。在示踪剂被困在大脑中后，大鼠被麻醉并用PET或SPECT扫描仪扫描。然后进行感兴趣体积分析和统计参数映射。我们可以在不接触老鼠的情况下注射示踪剂，同时保持它们的意识，直到示踪剂被分配并被困在大脑中。通过感兴趣体积和统计参数映射分析，观察氟西汀对葡萄糖摄取和脑血流量的影响。我们成功地开发了自由移动的18F-FDG-PET和99 mTc-HMPAO-SPECT成像系统，并检测了氟西汀给药后清醒大鼠脑中葡萄糖摄取和脑血流的详细变化。该系统有望用于在无麻醉和触觉刺激的情况下评估脑活动，以评估药物效果或动物脑功能。
The purpose of this study is the development of novel fluorine-18-fluorodeoxyglucose (18F-FDG)-PET and 99mTc-hexamethylpropylene amine oxime (HMPAO) SPECT methods with free-moving apparatus on conscious rats to investigate brain activity without the effects of anesthesia and tactual stimulation. We also assessed the sensitivity of the experimental system by an intervention study using fluoxetine as a reference drug. A catheter was inserted into the femoral vein and connected to a free-moving cannula system. After fluoxetine administration, the rats were given an injection of 18F-FDG or 99mTc-HMPAO via the intravenous cannula and released into a free-moving cage. After the tracer was trapped in the brain, the rats were anesthetized and scanned with PET or SPECT scanners. Then a volume of interest analysis and statistical parametric mapping were performed. We could inject the tracer without touching the rats, while keeping them conscious until the tracers were distributed and trapped in the brain using the developed system. The effects of fluoxetine on glucose uptake and cerebral blood flow were perceptively detected by volume of interest and statistical parametric mapping analysis. We successfully developed free-moving 18F-FDG-PET and 99mTc-HMPAO-SPECT imaging systems and detected detailed glucose uptake and cerebral blood flow changes in the conscious rat brain with fluoxetine administration. This system is expected to be useful to assess brain activity without the effects of anesthesia and tactual stimulation to evaluate drug effect or animal brain function.