Elicitation of primary and secondary metabolism during defense in the potato.
Elicitation of primary and secondary metabolism during defense in the potato.
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DOI：
10.1007/s10327-003-0075-6
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发表时间：
2003-12-01
影响因子：
1.2
通讯作者：
Yoshioka, Hirofumi
中科院分区：
文献类型：
--
作者：
Nakane, Eiichi;Kawakita, Kazuhito;Yoshioka, Hirofumi
马铃薯晚疫病菌(Phytophthora Infestans)侵染马铃薯块茎可引起多种防御反应，包括氧化爆发、苯丙烷类化合物和倍半萜类植物保卫素(lubimin和rishitin)的积累，并伴随产生它们的酶的从头合成。用从病原菌的菌丝体中制备的粗激发子(菌丝壁成分，或HWC)处理的马铃薯块茎也会发生这些反应。利用差异杂交技术从马铃薯块茎中鉴定了激发子反应的cDNA。数据库搜索显示，这些基因似乎编码酪胺羟基肉桂酰基转移酶、精氨酸脱羧酶、葡萄糖6-磷酸脱氢酶、3-脱氧-D-阿拉伯-7-磷酸合成酶、依赖于NADP的苹果酸酶、丙酮酸脱羧酶以及其他潜在的防御成分。RNA凝胶印迹分析表明，HWC激发子、花生四烯酸或水杨酸诱导了与次生代谢和初级代谢相关的转录本的积累。这表明，来自病原菌的信号不仅激活了次生代谢，还激活了支持马铃薯块茎快速表达防御反应的初级新陈代谢。
Exposure of potato tuber tissues to Phytophthora infestans, the late blight pathogen, elicits multiple defense responses, including an oxidative burst, accumulation of phenylpropanoid compounds, and sesquiterpenoid phytoalexins (lubimin and rishitin), which are accompanied by de novo synthesis of the enzymes that produce them. These reactions are also induced in potato tubers treated with a crude elicitor (hyphal wall components, or HWC) prepared from the mycelia of the pathogen. Differential hybridization was used to identify elicitor-responsive cDNAs from potato tubers. Database searches revealed that these genes appear to encode tyramine hydroxycinnamoyl transferase, arginine decarboxylase, glucose 6-phosphate dehydrogenase, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, NADP-dependent malic enzyme, pyruvate decarboxylase, and other potential defense components. RNA gel blot analyses indicated that the accumulation of transcripts related to both secondary and primary metabolism was induced by the HWC elicitor, arachidonic acid or salicylic acid. This suggests that the signals from the pathogen activate not only secondary metabolism but also the primary metabolism that supports the rapid expression of defense responses in potato tubers.