Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species
Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species
复制标题
DOI：
10.1038/s42003-019-0721-y
复制
发表时间：
2019-12-19
影响因子：
5.9
通讯作者：
Boraston, Alisdair B.
中科院分区：
文献类型：
--
作者：
Hettle, Andrew G.;Hobbs, Joanne K.;Boraston, Alisdair B.
假互单胞菌是一种全球分布的与海洋相关的属，可以在广泛的水生环境中发现，包括与大藻表面相关的大藻表面，在那里它们可以利用这些丰富的多糖来源。然而，赋予代谢这种丰富形式的光合作用固定碳的能力的代谢系统尚未完全了解。通过基因组学、转录组学、微生物学和途径组分的特定结构功能研究，我们研究了新分离的海洋假异交单胞菌代谢红藻半乳卡拉胶的能力。结果表明，kappa/iota-carrageenan特异性多糖利用位点（CarPUL）使具有该位点的菌株能够在该底物上生长。CarPUL酶组分的生化和结构分析促进了假异交单胞菌kappa/iota- carragean代谢途径的详细模型的发展，从而进一步了解这些微生物如何适应独特的环境生态位。
Pseudoalteromonas is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the kappa/iota-carrageenan specific polysaccharide utilization locus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the kappa/iota-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche.