The effects of freezing on the tensile properties of repaired porcine flexor tendon
The effects of freezing on the tensile properties of repaired porcine flexor tendon
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DOI：
10.1016/j.jhsa.2007.12.011
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发表时间：
2008-03-01
影响因子：
1.9
通讯作者：
O'Sullivan, Michael E.
中科院分区：
文献类型：
--
作者：
Hirpara, Kieran M.;Sullivan, Paulj.;O'Sullivan, Michael E.
目的：当进行复杂的测试时，必须充分保存样品以防止降解。冷冻是保存样品最方便的方法；然而，文献中没有证据证明在修复前后冷冻肌腱是可以接受的。我们的目的是证明冻结肌腱不会显著改变肌腱修复的线性载荷-失效测试结果。方法取150根猪前肢肌腱，随机分为5组，每组30根。分裂后，使用Pennington改良核心技术与Silfverskiold周围十字绣修复肌腱。1组在死后3小时内进行肌腱分离、修复和检测。2组肌腱在修复和测试前在4℃下冷藏24小时。第三组肌腱在-25℃冷冻3个月后进行修复和测试。第4组肌腱在修复和测试前在-25℃冷冻6个月。第5组肌腱在-25℃冷冻6个月，修复后再冷冻1个月，然后测试。所有修复体都进行了线性载荷测试，以确定修复体的极限强度和产生3mm间隙的力。结果方差分析结果未显示组间有显著性差异。结论在缝合修复前后冷冻肌腱是一种可接受的保存方法，可用于研究缝合修复前后产生3mm间隙的力和肌腱修复的极限强度。
Purpose When conducting complex testing often don repairs, it is essential that the samples are adequately preserved to prevent degradation. Freezing of samples is the most convenient method of preservation; however, there is no evidence in the literature to prove that freezing tendon before or after repair is acceptable. We aimed to prove that freezing tendons does not significantly alter the results of linear load-to-failure testing of tendon repairs.Methods After a power study, 150 tendons were harvested from porcine forelimbs and randomized into 5 groups of 30 tendons. After division, tendons were repaired using a Pennington modified core technique with a Silfverskiold peripheral cross-stitch. Tendons in group 1 were divided, repaired, and tested within 3 hours postmortem. Tendons in group 2 were refrigerated at 4 degrees C for 24 hours prior to repair and testing. Tendons in group 3 were frozen at -25 degrees C for 3 months prior to repair and testing. Tendons in group 4 were frozen at -25 degrees C for 6 months prior to repair and testing. Tendons in group 5 were frozen at -25 degrees C for 6 months, repaired, refrozen for 1 month, and then tested. All repairs were linear load tested to ascertain the ultimate strength and force to produce 3-mm gap in the repair.Results Analysis of variance analysis of the results did not demonstrate any significant differences between groups.Conclusions Freezing tendons both before and after suture repair is an acceptable method of preservation when investigating the force to produce 3-mm gap and ultimate strength of tendon repairs.