Isolation and characterization of rice phytochrome A mutants
Isolation and characterization of rice phytochrome A mutants
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DOI：
10.1105/tpc.13.3.521
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发表时间：
2001-03-01
期刊：
影响因子：
11.6
通讯作者：
Furuya, W
中科院分区：
文献类型：
--
作者：
Takano, M;Kanegae, H;Furuya, W
为了阐明光敏色素A（phyA）在水稻中的功能，我们通过聚合酶链反应筛选了大量反转录转座子（Tos 17）插入突变体，并分离出3个独立的phyA突变系。Tos17插入位点的测序证实，Tos17s中断PHYA基因的外显子在这些突变株系。此外，在这些phyA突变体中，phyA多肽不能被免疫化学检测到。phyA突变体在连续远红光下生长的幼苗表现出与黑暗生长的幼苗基本相同的表型，表明phyA突变体对远红光不敏感。phyA突变体的黄化幼苗对远红光或极低能量密度的红光脉冲也不敏感，相反，phyA突变体在连续红光下与野生型在形态上无法区分。因此，水稻phyA控制光形态建成在两个不同的模式的光感知远红光依赖的高辐照度响应和非常低的能量密度响应，这样的功能似乎是独特的，并限制在去黄化过程。有趣的是，连续远红光诱导水稻phyA幼苗中CAB和RBCS基因的表达，表明在黄化幼苗中存在除了phyA之外的能够感知连续远红光的光感受器。
To elucidate phytochrome A (phyA) function in rice, we screened a large population of retrotransposon (Tos17) insertional mutants by polymerase chain reaction and isolated three independent phyA mutant lines. Sequencing of the Tos17 insertion sites confirmed that the Tos17s interrupted exons of PHYA genes in these mutant lines. Moreover, the phyA polypeptides were not immunochemically detectable in these phyA mutants. The seedlings of phyA mutants grown in continuous far-red light showed essentially the same phenotype as dark-grown seedlings, indicating the insensitivity of phyA mutants to far-red light. The etiolated seedlings of phyA mutants also were insensitive to a pulse of far-red light or very low fluence red tight, In contrast, phyA mutants were morphologically indistinguishable from wild type under continuous red light. Therefore, rice phyA controls photomorphogenesis in two distinct modes of photoperception-far-red light-dependent high irradiance response and very low fluence response-and such function seems to be unique and restricted to the deetiolation process. interestingly, continuous far-red light induced the expression of CAB and RBCS genes in rice phyA seedlings, suggesting the existence of a photoreceptor(s) other than phyA that can perceive continuous far-red tight in the etiolated seedlings.