Oestrogen and progesterone receptor expression in the female lower urinary tract, with reference to oestrogen status
Oestrogen and progesterone receptor expression in the female lower urinary tract, with reference to oestrogen status
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DOI：
10.1046/j.1464-410x.2000.00724.x
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发表时间：
2000-07-01
影响因子：
4.5
通讯作者：
Bulmer, JN
中科院分区：
文献类型：
--
作者：
Blakeman, PJ;Hilton, P;Bulmer, JN
目的探讨雌激素受体和孕激素受体在女性下尿路中的分布和发生率，并比较不同雌激素水平的女性下尿路中雌激素受体和孕激素受体的表达。33例为绝经前患者，26例为绝经后患者，未补充雌激素，31例为绝经后患者，接受某种形式的雌激素替代治疗。在手术期间从膀胱顶、三角、近端尿道、远端尿道、阴道和膀胱颈区域的膀胱阴道筋膜进行活检。所有活检组织常规固定和处理的组织病理学评估，然后标记的单克隆抗体直接对人类雌激素（NCL-ERLH 2）和孕激素（NCL-PCR）受体。在所有组织的上皮、上皮下和肌肉/深筋膜区域中评估雌激素和孕激素受体表达，以确定每种受体的总体组织阳性，结果雌激素受体在所有女性下尿路鳞状上皮中均呈阳性表达，而在尿路上皮组织中均呈阴性表达与雌激素状态无关：组织学评分无显著变化。孕激素受体的表达是更多的变量，主要是subepithelial，并显着降低绝经后妇女没有接受雌激素replacement.Conclusion这些研究结果证实了女性下尿路是一个靶器官远的作用，雌激素和孕激素，并进一步阐明下尿路的地区可能会响应激素替代疗法。这可能对雌激素补充剂在治疗绝经后妇女下尿路疾病中的应用有影响。
Objective To assess the incidence and distribution of both oestrogen and progesterone receptors throughout the female lower urinary tract, and to compare receptor expression in women of varying oestrogen status.Patients and methods The study included 90 women undergoing surgery for urogynaecological conditions; 33 were premenopausal, 26 postmenopausal and taking no oestrogen supplementation, and 31 postmenopausal and receiving some form of hormone-replacement therapy. Biopsies were taken during surgery from the bladder dome, trigone, proximal urethra, distal urethra, vagina and vesicovaginal fascia in the region of the bladder neck. All biopsies were routinely fixed and processed for histopathological assessment and were then labelled immunohistochemically with monoclonal antibodies directed against human oestrogen (NCL-ERLH2) and progesterone (NCL-PGR) receptors. Both oestrogen and progesterone receptor expression were assessed in the epithelial, subepithelial and muscle/deeper fascial regions of all tissue for overall tissue positivity for each receptor, and by semiquantitative analysis of receptor concentration using histochemical scoring of the tissues.Results Oestrogen receptors were consistently detected in the squamous epithelia and were consistently absent in the urothelial tissues of the lower urinary tract of all women irrespective of oestrogen status: there was no significant variation in histological score. Progesterone receptor expression was more variable, being mostly subepithelial, and significantly lower in postmenopausal women receiving no oestrogen replacement.Conclusion These findings confirm the female lower urinary tract to be a target organ far the action of oestrogen and progesterone, and shed further light on the areas of the lower urinary tract likely to respond to hormone-replacement therapy. This may have implications for the use of oestrogen supplementation in the treatment of lower urinary tract disorders of postmenopausal women.