Anti-proliferative and mutagenic activities of aqueous and methanol extracts of leaves from Pereskia bleo (Kunth) DC (Cactaceae)
Anti-proliferative and mutagenic activities of aqueous and methanol extracts of leaves from Pereskia bleo (Kunth) DC (Cactaceae)
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DOI：
10.1016/j.jep.2007.06.026
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发表时间：
2007-09-25
影响因子：
5.4
通讯作者：
Radhakrishnan, Ammu Kutty
中科院分区：
文献类型：
--
作者：
Er, Hui Meng;Cheng, En-Hsiang;Radhakrishnan, Ammu Kutty
在最适（含10%胎牛血清（FBS）的培养基中）和次适（含0.5%FBS的培养基中）条件下，评价了Pereskia bleo（Kunth）DC（仙人掌科）叶的水提取物和甲醇提取物对小鼠乳腺癌细胞系（4 T1）和正常小鼠成纤维细胞系（NIH/3 T3）的抗增殖作用。在最佳条件下，水提取物在200 μ g/mL和300 μ g/mL下在4 T1细胞中以及在300 μ g/mL下在NIH/3 T3细胞中显示出显著的（p < 0.05）抗增殖作用，而甲醇提取物在任何测试浓度下在这些细胞系中均未显示出任何显著的抗增殖作用。在次优条件下，水提取物在200 μ g/mL和300 μ g/mL下在NIH/3 T3细胞中显示出显著（p <0.05）的抗增殖作用，而甲醇提取物在200 μ g/mL和300 μ g/mL下在两种细胞系中显示出显著（p < 0.05）的抗增殖作用。随着水提取物浓度的增加，4 T1和NIH/3 T3细胞的凋亡率呈上升趋势。在所有试验浓度的水提取物中观察到的细胞凋亡水平始终高于坏死。在用300 μ g/ML甲醇提取物处理的4 T1细胞中观察到的坏死水平显著增加（p < 0.05）。通常，在甲醇提取物处理的细胞中，注意到坏死的水平高于凋亡的水平。进行的致突变性试验表明，在不存在S-9肝脏代谢活化的情况下，浓度高达165 μ g/mL的浸提液无致突变性。然而，在存在S-9肝脏代谢活化的情况下，在所有检测浓度下，水提取物均具有致突变性。该研究表明，Pereskia bleo（Kunth）DC（仙人掌科）叶的水提取物和甲醇提取物对4 T1和NIH/3 T3细胞没有明显的抗增殖作用，因为当在最佳培养条件下测试时，获得的EC 50值大于50 μ g/mL。此外，水提取物可在肝酶代谢后形成诱变化合物。(C)2007爱思唯尔爱尔兰有限公司保留所有权利。
The anti-proliferative effects of the aqueous and methanol extracts of leaves of Pereskia bleo (Kunth) DC (Cactaceae) against a mouse mammary cancer cell line (4T1) and a normal mouse fibroblast cell line (NIH/3T3) were evaluated under an optimal (in culture medium containing 10% foetal bovine serum (FBS)) and a sub-optimal (in culture medium containing 0.5% FBS) conditions. Under the optimal condition, the aqueous extract showed a significant (p < 0.05) anti-proliferative effect at 200 mu g/mL and 300 mu g/mL in 4T1 cells and 300 mu g/mL in NIH/3T3 cells, whereas the methanol extract did not show any notable anti-proliferative effect in these cell lines, at any of the concentrations tested. Under the sub-optimal condition, the aqueous extract showed a significant (p < 0.05) anti-proliferative effect at 200 mu g/mL and 300 mu g/mL in NIH/3T3 cells, whilst the methanol extract showed a significant (p < 0.05) anti-proliferative effect at 200 mu g/mL and 300 mu g/mL in both cell lines. An upward trend of apoptosis was observed in both 4T1 and NIH/3T3 cells treated with increasing concentrations of the aqueous extract. The level of apoptosis observed at all the concentrations of the aqueous extract tested was consistently higher than necrosis. There was a significant (p < 0.05) increase in the level of necrosis observed in the 4T1 cells treated with 300 mu g/ML of the methanol extract. Generally, the level of necrosis was noted to be higher than that of apoptosis in the methanol extract-treated cells. The mutagenicity assay performed showed that in the absence of S-9 liver metabolic activation, the extract was not mutagenic up to the concentration of 165 mu g/mL. However, in the presence of S-9 liver metabolic activation, the aqueous extract was mutagenic at all the concentrations tested. This study shows that both the aqueous and methanol extracts of the leaves from Pereskia bleo (Kunth) DC (Cactaceae) do not have appreciable anti-proliferative effect on the 4T1 and NIH/3T3 cells as the EC50 values obtained are greater than 50 mu g/mL when tested under optimal culture condition. Moreover, the aqueous extract may form mutagenic compound(s) upon the metabolisation by liver enzymes. (C) 2007 Elsevier Ireland Ltd. All rights reserved.