Antiviral activity of Spirulina maxima against herpes simplex virus type 2
Antiviral activity of Spirulina maxima against herpes simplex virus type 2
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DOI：
10.1016/s0166-3542(02)00132-8
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发表时间：
2002-12-01
影响因子：
7.6
通讯作者：
Barron, BL
中科院分区：
文献类型：
--
作者：
Hernández-Corona, A;Nieves, I;Barron, BL
螺旋藻已被用于生物技术和医学科学的各种实际应用。本文介绍了在极大螺旋藻的商业制剂的热水提取物（HWE）中发现的抗病毒活性，通过使用几种病毒的微孔板抑制试验进行研究。HWE抑制单纯疱疹病毒2型（HSV-2）的感染。伪狂犬病病毒（PRV）、人巨细胞病毒（HCMV）和HSV-1的半数有效抑制量（ED_（50））为0.069。0.103、0.142和0.333 mg/ml。对于腺病毒，抑制小于20%，并且在2 mg/ml的HWE浓度下，未发现对麻疹病毒、亚急性硬化性全脑炎病毒（SSPE）、水泡性口炎病毒（VSV）、脊髓灰质炎病毒1和轮状病毒SA-11的抑制。抗病毒活性最高的是HSV-2，选择性指数为128。抗病毒活性不是由于杀病毒作用。疱疹病毒感染在病毒周期的初始事件（吸附和渗透）被抑制。初步分离和鉴定具有抗沙门氏菌活性的化合物。最大，一些提取物，使用几种不同极性的溶剂进行了评价，通过微孔板抑制试验，使用HSV-2。在甲醇-水3：1中检测到最高的抗病毒活性，这表明抗病毒活性可能是由于高极性化合物。(C)2002 Elsevier Science B. V.保留所有权利。
Spirulina has been used in a variety of practical applications in biotechnology and medical sciences. This paper presents the antiviral activity found in a hot water extract (HWE) of a commercial preparation of Spirulina maxima., studied by a microplate inhibition assay, using several viruses. The HWE inhibited the infection for: herpes simplex virus type 2 (HSV-2). pseudorabies virus (PRV), human cytomegalovirus (HCMV), and HSV-1, and the 50% effective inhibition doses (ED50) were 0.069. 0.103, 0.142, and 0.333 mg/ml for each virus, respectively. For adenovirus the inhibition was less than 20%, and no inhibition was found for measles virus, subacute sclerosing panencephalitis virus (SSPE), vesicular stomatitis virus (VSV), poliovirus 1 and rotavirus SA-11, at concentrations of 2 mg/ml of the HWE. The highest antiviral activity was for HSV-2, with a selectivity index of 128. The antiviral activity was not due to a virucidal effect. Herpesvirus infection was inhibited at the initial events (adsorption and penetration) of the viral cycle. To initiate the isolation and identification of the compound that exhibits the antiviral activity of S. maxima, some extracts made by using several solvents with different polarity were evaluated by microplate inhibition assay using HSV-2. The highest antiviral activity was detected in the methanol-water 3:1, which suggests that the antiviral activity is probably due to highly polar compounds. (C) 2002 Elsevier Science B.V. All rights reserved.