HPV typing and its relation with apoptosis in cervical carcinoma from Indian population
HPV typing and its relation with apoptosis in cervical carcinoma from Indian population
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DOI：
10.1007/s13277-011-0233-y
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发表时间：
2012-02-01
期刊：
影响因子：
--
通讯作者：
Rizvi, M. Moshahid Alam
中科院分区：
文献类型：
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作者：
Alam, M. Shabbir;Ali, Asgar;Rizvi, M. Moshahid Alam
在了解宫颈癌的病因方面已经取得了明确的进展，并且某些类型的人乳头瘤病毒已被确定为全世界宫颈癌的主要原因。本研究探讨宫颈鳞癌中人乳头瘤病毒感染及其与细胞凋亡和临床病理特征的关系。采用标准的聚合酶链反应方法，通过高危型人乳头瘤病毒的型特异性引物进行人乳头瘤病毒分型。通过末端脱氧核苷酸转移酶生物素-dUTP缺口末端标记法测定细胞程序性死亡（凋亡）。135例宫颈癌活检组织中131例（97%）检出人乳头瘤病毒感染。在人乳头瘤病毒阳性病例中，123例（94%）为人乳头瘤病毒16型，5例（4%）为人乳头瘤病毒18型。在135例宫颈癌中，81例（60%）出现凋亡。细胞凋亡现象在鳞癌中的发生率略高于腺癌（鳞癌40%，腺癌33%）。宫颈癌组织中人乳头瘤病毒感染可能与细胞凋亡的发生无关。
Definite progress in understanding the etiology of cervical cancer has been achieved, and some types of human papillomavirus have been established as the central cause of cervical cancer worldwide. This study investigates the human papillomavirus infection and its correlation with apoptosis and clinicopathologic characteristics in squamous cell carcinoma of uterine cervix. Human papillomavirus typing was done by type-specific primers for high-risk human papillomavirus using standard polymerase chain reaction method. Programmed cell death (apoptosis) was determined by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling assay. Human papillomavirus infection in tissue biopsy of cervical carcinoma was detected in 131 of 135 (97%) cases. Among the positive cases of human papillomavirus, 123 (94%) cases were human papillomavirus type 16, and five (4%) cases were human papillomavirus type 18. Out of 135 cervical carcinoma cases, 81 (60%) cases showed presence of apoptosis. The phenomenon of apoptosis was seen slightly higher in squamous cell carcinoma than in adenocarcinoma (40% in squamous cell carcinoma and 33% in adenocarcinoma). The human papillomavirus infection in cervical cancer might not play any role in the occurrence of apoptosis.