Hormonal regulation of growth hormone mRNA.
Hormonal regulation of growth hormone mRNA.
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生长激素 mRNA 的激素调节。
DOI：
10.1089/dna.1.1982.1.145
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Martial,JA
中科院分区：
文献类型：
--
作者：
Wegnez,M;Schachter,BS;Baxter,JD;Martial,JA
培养的大鼠垂体瘤细胞生长激素的产生受激素的调节。在以前的研究中，它表明，甲状腺和糖皮质激素刺激生长激素的合成。在目前的研究中，一个克隆的cDNA探针互补的生长激素mRNA被用来确定，通过溶液杂交，在各种激素条件下，每个细胞的生长激素mRNA分子的数量。用在三种不同培养基条件下生长的细胞进行实验，以区分添加的激素的作用与血清因子的作用。结果表明，在72小时内，甲状腺激素和糖皮质激素，单独添加时，能够诱导细胞质生长激素mRNA的显着积累。然而，为了获得最大的效率，这两种激素有不同的要求：糖皮质激素似乎只需要甲状腺激素的存在，而甲状腺激素似乎需要一些尚未确定的血清因子。当这两类激素加在一起时，会产生协同作用。这些细胞中生长激素mRNA的最大范围变化超过500倍，从每个细胞约1000个分子（10%小牛血清加甲状腺和糖皮质激素）到每个细胞不到2个分子（在不添加激素的含血清替代品的培养基中）。这些数据进一步表明，在这些GH 3细胞中，生长激素mRNA的翻译效率也可以变化（4倍），这取决于所使用的激素条件。然而，这些后者的影响是不特定的生长激素mRNA，而是出现在所有的mRNA群体的一般翻译效果。这些结果表明，甲状腺激素和糖皮质激素对生长激素产生的主要影响是由于对生长激素mRNA分子数量的影响。
Production of growth hormone by cultured rat pituitary tumor cells (GH3subline) is regulated by hormones. In previous studies, it was shown that thyroid and glucocorticoid hormones stimulate growth hormone synthesis. In the current studies, a cloned cDNA probe complementary to growth hormone mRNA was used to determine, by solution hybridization, the number of growth hormone mRNA molecules per cell under various hormonal conditions. The experiments were performed with cells grown in three different medium conditions to distinguish the effect of the added hormones from the effect of serum factors. The results show that, within 72 h, both thyroid and glucocorticoid hormones, when added alone, are able to induce a significant accumulation of cytoplasmic growth hormone mRNA. However, to obtain their maximal efficiencies, the two types of hormones have different requirements: glucocorticoids appear to need only the presence of thyroid hormones, whereas thyroid hormones appear to require some as yet unidentified serum factors. When added together, the two classes of hormones act synergistically. The maximal range of growth hormone mRNA in these cells varies by over 500-fold—from about 1000 molecules per cell (10% calf serum plus thyroid and glucocorticoid hormones) to less than two molecules per cell (in a defined serum-substitute-containing medium with no hormone added). The data further suggest that, in these GH3cells, the efficiency of translation of growth hormone mRNA can also vary (by a factor of 4), depending on the hormonal conditions used. However, these latter influences are not specific for growth hormone mRNA but rather appear to be a general translational effect on all the mRNA population. These results demonstrate that the major effect of thyroid and glucocorticoid hormones on growth hormone production is due to effects on the number of growth hormone mRNA molecules.