Two sRNA RyhB homologs from Yersinia pestis biovar microtus expressed in vivo have differential Hfq-dependent stability
Two sRNA RyhB homologs from Yersinia pestis biovar microtus expressed in vivo have differential Hfq-dependent stability
复制标题
鼠疫耶尔森氏菌体内表达的两种 sRNA RyhB 同源物具有不同的 Hfq 依赖性稳定性
DOI：
10.1016/j.resmic.2012.05.006
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发表时间：
2012-07-01
影响因子：
2.6
通讯作者：
Han, Yanping
中科院分区：
文献类型：
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作者：
Deng, Zhongliang;Meng, Xiangrong;Han, Yanping
小非编码 RNA (sRNA) 已被证明可以在转录后水平调节基因表达。 RyhB 是一种铁响应性 sRNA，在大肠杆菌和其他肠杆菌中保守，表明铁耗竭期间许多基因的下调。该 sRNA 受到铁摄取调节因子 (Fur) 的严格调节，并与 RNA 结合蛋白 Hfq 相互作用。一般认为 Hfq 对于稳定 sRNA 和促进 sRNA-mRNA 双链体形成至关重要。维持铁稳态是鼠疫耶尔森氏菌生命周期中的一个重要步骤。鼠疫耶尔森氏菌编码两种 RyhB 同源物：RyhB1 和 RyhB2。在这项研究中，我们发现与大肠杆菌一样，鼠疫耶尔森氏菌中的两种 RyhB 同源物均受到 Fur 的负调控，并且半衰期 > 30 分钟。在没有 Hfq 的情况下，RyhB1 会迅速降解，而 RyhB2 则保持其稳定性。 RyhB I 的稳定性是由 Hfq 介导的，但 RyhB2 不需要 Hfq 来实现稳定性。此外，两种 RyhB 在感染鼠疫耶尔森氏菌的肺部中均上调，而 ryhB 突变体在皮下或鼻内接种后对小鼠的毒力没有明显影响。总的来说，我们的结果表明，两种 RyhB 同源物在鼠疫耶尔森氏菌感染的肺部和体外具有共同的调节特征，但 RyhB1 和 RyhB2 的稳定性对 Hfq 的依赖性不同。 (c) 2012 年巴斯德研究所。由 Elsevier Masson SAS 出版。版权所有。
Small non-coding RNAs (sRNAs) have been shown to modulate gene expression at the post-transcriptional level. RyhB, an iron-responsive sRNA, is conserved in Escherichia coli and other Enterobacteriae, indicating the downregulation of numerous genes during iron depletion. This sRNA is tightly regulated by the ferric uptake regulator (Fur) and interacts with the RNA binding protein Hfq. Hfq is generally purported to be essential for stabilizing sRNAs and promoting sRNA-mRNA duplex formation. Maintenance of iron homeostasis is an essential step in the lifecycle of Yersinia pestis. Y. pestis encodes two RyhB homologs, RyhB1 and RyhB2. In this study, we found that as in the case of E. colt, both RyhB homologs in Y. pestis are negatively regulated by Fur and have a half-life of >30 min. In the absence of Hfq, RyhB1 is rapidly degraded, while RyhB2 retains its stability. RyhB I stabilization is mediated by Hfq, but RyhB2 does not require Hfq for stability. Additionally, both RyhBs are upregulated in lungs infected with Y. pestis, while the ryhB mutant shows no visible effects on virulence in mice upon either subcutaneous or intranasal inoculation. Collectively, our results indicate that the two RyhB homologs have common regulatory features in Y. pestis-infected lungs and in vitro, but that stability of RyhB1 and RyhB2 is differentially dependent on Hfq. (c) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.