Licorice root extract and magnesium isoglycyrrhizinate protect against triptolide-induced hepatotoxicity via up-regulation of the Nrf2 pathway.
Licorice root extract and magnesium isoglycyrrhizinate protect against triptolide-induced hepatotoxicity via up-regulation of the Nrf2 pathway.
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甘草根提取物和异甘草酸镁通过上调 Nrf2 通路防止雷公藤甲素诱导的肝毒性
DOI：
10.1080/10717544.2018.1472676
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发表时间：
2018-11
期刊：
影响因子：
6
通讯作者：
Jin J
中科院分区：
文献类型：
--
作者：
Tan QY;Hu Q;Zhu SN;Jia LL;Xiao J;Su HZ;Huang SY;Zhang J;Jin J
摘要雷公藤甲素是中药雷公藤的主要生物活性成分，具有多种药理活性，包括抗炎、抗生育、抗肿瘤和免疫抑制作用。然而，毒性和严重的不良反应，特别是肝毒性，限制了雷公藤甲素的临床应用。甘草根提取物含有多种生物活性化合物，具有强效保肝作用。异甘草酸镁是甘草酸的18α-甘草酸立体异构体的镁盐，在中国临床上用于治疗慢性病毒性肝炎和急性药物性肝损伤。本研究的目的是探讨红细胞2因子相关因子2途径的作用，LE和雷公藤甲素对雷公藤甲素诱导的肝毒性的保护作用。采用雷公藤甲素建立L-02细胞和大鼠肝毒性模型，分别在体内外观察LE和雷公藤甲素对肝毒性的保护作用。LE和ESTO对雷公藤甲素诱导的细胞毒性有显著的保护作用。此外，雷公藤内酯醇降低了Nrf 2的mRNA和蛋白水平，下调了Nrf 2靶基因，包括UGT 1A，BSEP和MRP 2，而用LE和ESTA预处理逆转了这些作用。最后，Nrf 2参与的抗氧化反应被激活的存在下，LE和ESTA。
Abstract Triptolide, the predominant biologically active component of the Chinese herb Tripterygium wilfordii Hook f., possesses numerous pharmacological activities, including anti-inflammatory, anti-fertility, anti-neoplastic, and immunosuppressive effects. However, toxicity and severe adverse effects, particularly hepatotoxicity, limit the clinical application of triptolide. Licorice root extract contains various bioactive compounds and is potent hepatoprotective. Magnesium isoglycyrrhizinate, a magnesium salt of the 18α-glycyrrhizic acid stereoisomer of glycyrrhizic acid, is used clinically in China to treat chronic viral hepatitis and acute drug-induced liver injury. The aim of this study was to investigate the role of the factor erythroid 2-related factor 2 pathway in the protective effects of LE and MIG against triptolide-induced hepatotoxicity. Hepatotoxicity models were established in L-02 cells and rats using triptolide, and the protective effects of LE and MIG were investigated in vitro and in vivo, respectively. LE and MIG significantly protected against triptolide-induced cytotoxicity. Additionally, triptolide decreased the mRNA and protein levels of Nrf2 and down-regulated Nrf2 target genes, including UGT1A, BSEP, and MRP2, while pretreatment with LE and MIG reversed these effects. Finally, Nrf2-involved antioxidant responses were activated in the presence of LE and MIG.
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影响因子：
2.9
作者：
Lam, P;Wang, RX;Ling, V
通讯作者：
Ling, V
p62-Keap1-NRF2 通路的激活可防止肝细胞癌细胞中的铁死亡。
DOI：
10.1002/hep.28251
发表时间：
2016-01
期刊：
Hepatology (Baltimore, Md.)
影响因子：
--
作者：
Sun X;Ou Z;Chen R;Niu X;Chen D;Kang R;Tang D
通讯作者：
Tang D
Nrf2 的激活可防止雷公藤甲素诱发的肝毒性
影响因子：
3.7
作者：
Li J;Shen F;Guan C;Wang W;Sun X;Fu X;Huang M;Jin J;Huang Z
通讯作者：
Huang Z
影响因子：
4.3
作者：
Qu, Xiao-Yu;Tao, Li-Na;Song, Yan-Qing
通讯作者：
Song, Yan-Qing
影响因子：
6.1
作者：
Fan Yuan-Jing;Wei Wei;Dai Zi-Ling
通讯作者：
Dai Zi-Ling