Differential Expression of Mitosis and Cell Cycle Regulatory Genes during Recovery from an Acute Respiratory Virus Infection.
Differential Expression of Mitosis and Cell Cycle Regulatory Genes during Recovery from an Acute Respiratory Virus Infection.
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急性呼吸道病毒感染恢复过程中有丝分裂和细胞周期调控基因的差异表达。
DOI：
10.3390/pathogens10121625
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发表时间：
2021-12-15
期刊：
影响因子：
--
通讯作者：
Rosenberg HF
中科院分区：
文献类型：
--
作者：
Limkar AR;Lack JB;Sek AC;Percopo CM;Druey KM;Rosenberg HF
急性呼吸道病毒感染可能对肺功能产生深远而长期的影响，即使在急性反应完全消退后也会持续存在。在这项研究中，我们研究了野生型BALB/c小鼠的肺组织中的基因表达的RNA测序，从小鼠肺炎病毒（PVM）的亚致死性感染中恢复，这是一种与人类呼吸道合胞病毒相同的病毒家族和属的天然啮齿动物病原体。我们比较了用植物乳杆菌治疗的PVM感染小鼠的基因表达的这些反应，植物乳杆菌是一种免疫生物制剂，可以限制炎症并避免通常在急性感染这种病原体时观察到的负面临床后遗症。我们的研究结果揭示了炎症相关基因以及与有丝分裂和细胞周期调控有关的许多基因和基因家族的显著差异表达，包括细胞周期蛋白、细胞周期蛋白依赖性激酶、细胞分裂周期基因、E2 F转录因子、驱动蛋白、着丝粒蛋白和极光激酶等。特别值得注意的是细胞分裂周期基因Cdc 20 b的差异表达，其先前被鉴定为对多纤毛细胞的离体分化至关重要。总的来说，这些发现为我们提供了对病毒后修复过程的实质性了解，并拓宽了我们对乳杆菌介导的保护机制的理解。
Acute respiratory virus infections can have profound and long-term effects on lung function that persist even after the acute responses have fully resolved. In this study, we examined gene expression by RNA sequencing in the lung tissue of wild-type BALB/c mice that were recovering from a sublethal infection with the pneumonia virus of mice (PVM), a natural rodent pathogen of the same virus family and genus as the human respiratory syncytial virus. We compared these responses to gene expression in PVM-infected mice treated with Lactobacillus plantarum, an immunobiotic agent that limits inflammation and averts the negative clinical sequelae typically observed in response to acute infection with this pathogen. Our findings revealed prominent differential expression of inflammation-associated genes as well as numerous genes and gene families implicated in mitosis and cell-cycle regulation, including cyclins, cyclin-dependent kinases, cell division cycle genes, E2F transcription factors, kinesins, centromere proteins, and aurora kinases, among others. Of particular note was the differential expression of the cell division cycle gene Cdc20b, which was previously identified as critical for the ex vivo differentiation of multi-ciliated cells. Collectively, these findings provided us with substantial insight into post-viral repair processes and broadened our understanding of the mechanisms underlying Lactobacillus-mediated protection.
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影响因子：
4.4
作者：
Hirano T
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影响因子：
11.4
作者：
Holcomb, IN;Kabakoff, RC;Hébert, CC
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DOI：
10.1152/ajplung.00202.2009
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2010-11-01
影响因子：
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