Genetic dissection and pyramiding of quantitative traits for panicle architecture by using chromosomal segment substitution lines in rice
Genetic dissection and pyramiding of quantitative traits for panicle architecture by using chromosomal segment substitution lines in rice
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DOI：
10.1007/s00122-008-0722-6
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发表时间：
2008-04-01
影响因子：
5.4
通讯作者：
Yano, Masahiro
中科院分区：
文献类型：
--
作者：
Ando, Tsuyu;Yamamoto, Toshio;Yano, Masahiro
为了了解水稻产量相关性状的遗传基础，我们从一个平均产量的粳稻品种，Sasanishiki，作为轮回亲本和一个高产的籼稻品种，Habataki，作为供体之间的杂交培育了39个染色体片段置换系（CSSLs）。在2年的时间里，对5个穗部形态性状的CSSLs进行了检测，共检测到38个数量性状位点，分布在11条染色体上。每个QTL的加性效应相对较小，表明没有一个QTL可以解释Sasanishiki和Habataki之间库大小的表型差异。我们开发了两个主要QTL的近等基因系，qSBN1（第1染色体上的二次分支数）和qPBN6（第6染色体上的一次分支数），并含有这两个线。这些线的表型分析表明，qSBN1和qPBN6独立贡献库大小和组合线产生更多的小穗。这表明分布在整个基因组中的QTL的累积效应构成了水稻穗构型的主要遗传基础。
To understand the genetic basis of yield-related traits of rice, we developed 39 chromosome segment substitution lines (CSSLs) from a cross between an average-yielding japonica cultivar, Sasanishiki, as the recurrent parent and a high-yielding indica cultivar, Habataki, as the donor. Five morphological components of panicle architecture in the CSSLs were evaluated in 2 years, and 38 quantitative trait loci (QTLs) distributed on 11 chromosomes were detected. The additive effect of each QTL was relatively small, suggesting that none of the QTLs could explain much of the phenotypic difference in sink size between Sasanishiki and Habataki. We developed nearly isogenic lines for two major QTLs, qSBN1 (for secondary branch number on chromosome 1) and qPBN6 (for primary branch number on chromosome 6), and a line containing both. Phenotypic analysis of these lines revealed that qSBN1 and qPBN6 contributed independently to sink size and that the combined line produced more spikelets. This suggests that the cumulative effects of QTLs distributed throughout the genome form the major genetic basis of panicle architecture in rice.