ABA Controls H2O2 Accumulation Through the Induction of OsCATB in Rice Leaves Under Water Stress
ABA Controls H2O2 Accumulation Through the Induction of OsCATB in Rice Leaves Under Water Stress
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ABA 通过诱导水分胁迫下水稻叶片中的 OsCATB 来控制 H2O2 积累
DOI：
10.1093/pcp/pcr028
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发表时间：
2011-04-01
影响因子：
4.9
通讯作者：
Zhang, Jianhua
中科院分区：
文献类型：
--
作者：
Ye, Nenghui;Zhu, Guohui;Zhang, Jianhua
ABA和H2O2的产生都是由干旱诱导的，在胁迫条件下可以作为信号。研究了聚乙二醇胁迫下水稻叶片中ABA、H2O2和过氧化氢酶（CAT）的关系。OsNCED3作为ABA生物合成的关键基因，在水分胁迫处理下被显著诱导，并先于ABA的快速增加。水分胁迫抑制了CATA和CATC的表达，但显著增强了CATB的表达。外源ABA也促进了CATC的表达，并呈浓度依赖性抑制CATC的表达。当使用ABA生物合成抑制剂去二氢愈创木酸和钨酸盐抑制ABA的产生时，水分胁迫下CATB的表达也受到抑制，而CATC的表达则得到增强。当内源ABA的产生受到抑制时，H2O2的积累也会减少，并与过氧化氢酶的总活性呈正相关。我们的研究结果表明，水分胁迫诱导的ABA通过诱导CATB基因在水分胁迫下的表达来阻止H2O2的过度积累。
The production of both ABA and H2O2 is induced by drought and can act as signals under stress conditions. We investigated the relationships between ABA, H2O2 and catalase (CAT) in rice leaves when rice seedlings were treated with polyethylene glycol as water stress treatment. As a key gene in ABA biosynthesis, OsNCED3 was significantly induced in rice by water stress treatment and such induction preceded the rapid increase in ABA. Water stress inhibited the expression of CATA and CATC but substantially enhanced the expression of CATB. Exogenously applied ABA promoted the expression of CATB also and inhibited the expression of CATC in a concentration-dependent manner. When ABA production was inhibited by using ABA biosynthesis inhibitors nordihydroguaiaretic acid and tungstate, expression of CATB was also subdued while CATC was enhanced under the water stress. Accumulation of H2O2 was also reduced when endogenous ABA production was inhibited and showed a correlation with the total activity of catalases. Our results suggest that water stress-induced ABA prevents the excessive accumulation of H2O2, through the induction of the expression of CATB gene during water stress.