Actin gene structure in twoArtemia species,A. franciscana andA. parthenogenetica
Actin gene structure in twoArtemia species,A. franciscana andA. parthenogenetica
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两种卤虫的肌动蛋白基因结构，A.
DOI：
10.1007/bf02338830
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发表时间：
1996
影响因子：
3.9
通讯作者：
L. Sastre
中科院分区：
文献类型：
--
作者：
M. Ortega;M. Díaz;L. Sastre
编码肌动蛋白的基因组克隆已从两种甲壳类卤虫中分离出来。孤雌生殖和A.弗朗西斯卡纳。 Act211同工型基因是从A.parthenogenica中分离出来的，另外两个同工型基因Act302和Act403是从A.parthenogenica中分离出来的。弗朗西斯卡纳。基因组和 cDNA 克隆的核苷酸序列的比较显示，翻译区域的种间差异为 4%，非翻译区域的种间差异为 6.1%。然而，A中Act211基因的部分结构的建立。 franciscana 和 A 中的 Act302 基因。单性生殖表明这两个物种的相似性。 Act211基因分为四个外显子，Act302基因分为六个外显子，Act403基因分为七个外显子。这三个基因在 5' 非翻译区和密码子 41 和 42 之间有一个内含子。Act211 和 403 基因在密码子 168 中有一个共同的内含子。Act302 和 403 基因在密码子 121-122、246-247 和密码子 301 之间有共同的内含子。而在 5' 非翻译区和密码子之间有一个共同的内含子。密码子41-42和121-122存在于许多生物体中，位置168和246-247的内含子之前仅分别在线虫盘尾丝虫和绿藻Volvox carterii的肌动蛋白基因中发现。 301位的内含子以前没有报道过。这三个基因的转录起始位点以及启动子区域的核苷酸序列也已确定。
Genomic clones coding for actin have been isolated from two species of the crustaceanArtemia,A. parthenogenetica andA. franciscana. The Act211 isoform gene was isolated from A.parthenogenetica, and the two other isoform genes, Act302 and Act403, were isolated fromA. franciscana. The comparison of the nucleotide sequence of genomic and cDNA clones showed an interspecific divergence of 4% in translated and 6.1% in untranslated regions. However, the establishment of the partial structure of the Act211 gene inA. franciscana and of the Act302 gene inA. parthenogenetica suggests their similarity in the two species. The Act211 gene is divided into four exons, the Act302 gene into six exons, and the Act403 gene into seven exons. The three genes have introns in the 5′ untranslated region and between codons 41 and 42. The Act211 and 403 genes have one common intron in codon 168. The Act302 and 403 genes have common introns between codons 121–122, 246–247, and within codon 301. While introns in the 5′ untranslated region and between codons 41–42 and 121–122 are present in many organisms, the introns in positions 168 and 246–247 had only been found previously in actin genes from the nematodeOnchocerca volvulus and the green algaVolvox carterii, respectively. The intron in position 301 had not been reported before. The transcription initiation sites of these three genes as well as the nucleotide sequences of the promoter regions have been also determined.
影响因子：
5.3
作者：
GOSSETT, LA;KELVIN, DJ;OLSON, EN
通讯作者：
OLSON, EN