Pig Ascaris: an important source of human ascariasis in China.
Pig Ascaris: an important source of human ascariasis in China.
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DOI：
10.1016/j.meegid.2012.04.016
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发表时间：
2012-08
期刊：
影响因子：
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通讯作者：
Chunhua Zhou;Min Li;K. Yuan;Shoulong Deng;W. Peng
中科院分区：
文献类型：
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作者：
Chunhua Zhou;Min Li;K. Yuan;Shoulong Deng;W. Peng
本研究的目的是检测中国人猪蛔虫交叉感染和杂交的频率和分布。选择20个高多态性微卫星位点，筛选来自中国6个省份的人和猪的258种蛔虫。使用Structure、Baps和Newhybrids软件确定人与猪蛔虫交叉感染和杂交的情况。结果显示，所有采样点均发现交叉感染，20例交叉感染病例中，19例为人感染纯种猪蛔虫，而只有1例为猪感染纯种人型蛔虫。与交叉感染的结果类似，在所有地点也都检测到了杂交蛔虫，并且两种宿主物种和大多数杂交蛔虫（95%）都是从人类宿主中检测到的。交叉感染和杂种的分布在两种寄主物种之间、G1、G2和G3三类基因型之间以及南北地区之间（仅针对杂种）均表现出显着差异。结果强烈表明，在人蛔虫和猪蛔虫流行区，猪蛔虫是人类蛔虫病的重要来源。考虑到目前人类蛔虫病的控制措施仅针对感染者，迫切需要修改现有的控制措施，增加对同域流行的感染猪的同步治疗。人与猪蛔虫交叉传播和杂交的知识不仅对于公共卫生具有重要意义，而且对于了解蛔虫的遗传进化、分类学和分子流行病学也具有重要意义。
The aim of the present study is to detect the frequency and distribution of cross infection and hybridization of human and pig Ascaris in China. Twenty high polymorphic microsatellite loci were selected to screen 258 Ascaris worms from humans and pigs from six provinces in China. The software programs Structure, Baps and Newhybrids were used to determine the case of cross infection and hybridization of human and pig Ascaris. Results showed that cross infection was detected in all sampled locations and of the total 20 cross infection cases, 19 were indentified as human infections by pure-bred pig type Ascaris in contrast to only one case of pig infection by pure-bred human type Ascaris. Similar to the findings in cross infection, hybrid Ascaris was also detected in all locations and both host species and most of hybrids (95%) were detected from human host. The distribution of cross infection and hybrids showed significant difference between the two host species and among three categories of genotype in terms of G1, G2 and G3, and also between the south and north regions (for hybrids only). The results strongly suggest pig Ascaris as an important source of human ascariasis in endemic area where both human and pig Ascaris exist. In consideration of current control measures for human ascariasis targeting only infected people, it is urgently needed to revise current control measures by adding a simultaneous treatment to infected pigs in the sympatric endemics. The knowledge on cross transmission and hybridization between human and pig Ascaris is important not only for public health, but also for the understanding of genetic evolution, taxonomy and molecular epidemiology of Ascaris.