Evidence of two distinct subsubtypes within the HIV-1 subtype A radiation
Evidence of two distinct subsubtypes within the HIV-1 subtype A radiation
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DOI：
10.1089/088922201750236951
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发表时间：
2001-05-20
影响因子：
1.5
通讯作者：
Peeters, M
中科院分区：
文献类型：
--
作者：
Gao, F;Vidal, N;Peeters, M
HIV-1 M组成员是世界上绝大多数艾滋病病例的罪魁祸首，并根据它们的系统发育关系被分为九个大致等距的分支，称为亚型。虽然这些基因型的表型相关性尚不清楚，但这些谱系中某些谱系的不成比例分布表明可能存在亚型特有的生物学差异，因此亚型命名法仍然是追踪M组大流行过程的重要分子流行病学工具。在本研究中，我们根据部分序列分析将先前描述的HIV-1毒株表征为不寻常的A亚型变异株，其中6株来自塞浦路斯(CY)，从韩国(KR)和刚果民主共和国(CD)的感染细胞培养物或患者PBMC DNA中扩增、克隆和全序列(94CY017、97KR004、97CDKTB48和97CDKP58)或半基因组(97CDKS10和97CDKFE4)，距离和系统发育分析表明，这些病毒中的4株(94CY017、97CDKTB48、97CDKFE4和97CDKS10)彼此关系密切，但与所有其他HIV-1毒株(代表它们最近的亲缘关系的A亚型病毒除外)有很大的差异。在Gag区、Pol区、env区和nef区的系统发育树中，这四个新鉴定的HIV-1毒株与A亚型形成了一个不同的姐妹分支，与A亚型的亲缘关系就像F1和F2亚型之间的亲缘关系一样密切。根据目前的命名规则，这定义了一个亚型，我们暂时将其命名为A2亚型，另外两种病毒97KR004和97CDKP58以及来自序列数据库(ZAM184)的全长HIV-1序列分别代表复杂的A2/D、A2/G和A2/C重组体。这些结果表明，HIV-1 A亚型由两个亚型(A1和A2)组成，这两个亚型似乎都有广泛的地理分布。这里描述的A2病毒代表了这一新的M组谱系的第一批参考试剂。
Members of HIV-1 group M are responsible for the vast majority of AIDS cases worldwide and have been classified on the basis of their phylogenetic relationships into nine roughly equidistant clades, termed subtypes, Although there are no known phenotypic correlates for these genotypes, the disproportionate spread of certain of these lineages has been taken to indicate that subtype-specific biological differences may exist, The subtype nomenclature thus remains an important molecular epidemiological tool with which to track the course of the group M pandemic, In this study, we have characterized HIV-1 strains described previously as unusual subtype A variants on the basis of partial sequence analysis, Six such strains from Cyprus (CY), South Korea (KR), and the Democratic Republic of Congo (CD) were PCR amplified from infected cell culture or patient PBMC DNA, cloned, and sequences in their entirety (94CY017, 97KR004, 97CDKTB48, and 97CDKP58) or as half genomes (97CDKS10 and 97CDKFE4), Distance and phylogenetic analyses showed that four of these viruses (94CY017, 97CDKTB48, 97CDKFE4, and 97CDKS10) were closely related to each other, but quite divergent from all other HIV-1 strains, except for subtype A viruses, which represented their closest relatives. In phylogenetic trees from gag, pol, env, and nef regions, the four newly characterized HIV-1 strains formed a distinct sister clade to subtype A, which was as closely related to subtype A as subsubtypes F1 and F2 are to each other. According to current nomenclature rules, this defines a subsubtype, which we have tentatively termed A2, The two other viruses, 97KR004 and 97CDKP58, as well as a full-length HIV-1 sequence from the sequence database (ZAM184), were found to represent complex A2/D, A2/G, and A2/C recombinants, respectively, These results indicate that HIV-1 subtype A is composed of two subsubtypes (A1 and A2), both of which appear to have a widespread geographic distribution. The A2 viruses described here represent the first reference reagents for this new group M lineage.