Focal adhesions are involved in simulated-microgravity-induced basilar and femoral arterial remodelling in rats
Focal adhesions are involved in simulated-microgravity-induced basilar and femoral arterial remodelling in rats
复制标题
粘着斑参与模拟微重力诱导的大鼠基底动脉和股动脉重塑
DOI：
10.1139/cjpp-2017-0665
复制
发表时间：
2018
影响因子：
2.1
通讯作者：
Ma Jin
中科院分区：
文献类型：
--
作者：
Jiang Min;Lyu Qiang;Bai YunGang;Liu Huan;Yang Jing;Cheng JiuHua;Zheng Ming;Ma Jin
最近的研究表明，微重力诱导的动脉重塑导致飞行后立位耐力障碍，多种机制参与了动脉重塑。然而，血流动力学改变导致动脉重构的最初机制尚不清楚。焦点粘连(FAs)是一种具有机械转导特性的动态蛋白质复合体。本研究旨在探讨FAs在模拟微重力诱导的基底动脉和股动脉重构中的作用。采用4周后肢失重(HU)大鼠模型模拟微重力效应，每日1h间歇性人工重力(IAG)预防动脉重塑。HU后4周，基底动脉管壁厚度、平滑肌细胞体积和胶原含量增加，股动脉减少(P<；0.05)。此外，HU还可上调和下调p-FAK Y397和p-Src Y418在基底动脉和股动脉的表达(P<；0.05)。HU使基底动脉FAs数增加，股动脉FAs数减少(P<；0.05)。此外，每天1h的IAG可阻止HU诱导的不同结构适应和基底动脉和股动脉FAs的变化。这些结果表明，FAs可能通过启动细胞内信号转导来响应微重力引起的机械应力变化，从而在动脉重塑中发挥机械传感器的作用。
Recent studies have suggested that microgravity-induced arterial remodelling contributes to post-flight orthostatic intolerance and that multiple mechanisms are involved in arterial remodelling. However, the initial mechanism by which haemodynamic changes induce arterial remodelling is unknown. Focal adhesions (FAs) are dynamic protein complexes that have mechanotransduction properties. This study aimed to investigate the role of FAs in simulated-microgravity-induced basilar and femoral arterial remodelling. A 4-week hindlimb-unweighted (HU) rat model was used to simulate the effects of microgravity, and daily 1-hour intermittent artificial gravity (IAG) was used to prevent arterial remodelling. After 4-week HU, wall thickness, volume of smooth muscle cells (SMCs) and collagen content were increased in basilar artery but decreased in femoral artery (P< 0.05). Additionally, the expression of p-FAK Y397 and p-Src Y418 was increased and reduced in SMCs of basilar and femoral arteries, respectively, by HU (P< 0.05). The number of FAs was increased in basilar artery and reduced in femoral artery by HU (P< 0.05). Furthermore, daily 1-hour IAG prevented HU-induced differential structural adaptations and changes in FAs of basilar and femoral arteries. These results suggest that FAs may act as mechanosensors in arterial remodelling by initiating intracellular signal transduction in response to altered mechanical stress induced by microgravity.