Influence of synthetic somatostatin upon growth hormone release from perifused rat pituitaries.
Influence of synthetic somatostatin upon growth hormone release from perifused rat pituitaries.
复制标题
合成生长抑素对灌注大鼠垂体释放生长激素的影响。
DOI：
10.1210/endo-99-3-678
复制
发表时间：
1976
期刊：
影响因子：
4.8
通讯作者：
M. Stachura
中科院分区：
文献类型：
--
作者：
M. Stachura
从灌注的大鼠垂体外植体中释放预先标记的、储存的氚化大鼠生长激素（[3H]rGH）的基础是垂体[LH]rGH含量的恒定部分。合成生长抑素（SRIF）以剂量依赖性方式抑制预先标记的储存[3H]rGH的释放。长时间暴露于SRIF导致[3H]rGH释放的立即和持续抑制。在体外灌流系统中，最大抑制，这是实现与25 nM SRIF，结果在[3H]rGH释放率是基础释放的30%至40%。SRIF脉冲对[3H]rGH释放产生抑制，随后在SRIF停药后出现反弹释放。当暴露于SRIF的时间保持恒定时，SRIF诱导的抑制和[3H]rGH的反弹释放都具有剂量依赖性。当恒定的SRIF浓度脉冲可变的时间段时，观察到类似的进行性反应。SRIF脉冲对[3H]rGH释放无净抑制作用。
Basal release of pre-labeled, stored tritiated rat growth hormone ([3H]rGH) from perfused rat pituitary explants is a constant fraction of pituitary [LH]rGH content. Synthetic somatostatin (SRIF) inhibits the release of pre-labeled, stored [3H]rGH in a dose-dependent fashion. Prolonged exposure to SRIF results in an immediate and continuous inhibition of [3H]rGH release. In the in vitro perifusion system, the maximal inhibition, which is achieved with 25 nM SRIF, results in a rate of [3H]rGH release which is 30 to 40 per cent of basal release. Pulses of SRIF produce inhibition of [3H]rGH release followed by rebound release after withdrawal of SRIF. When the time of exposure to SRIF is held constant, both the SRIF-induced inhibition and the rebound release of [3H]rGH are dose-dependent. A similar progressive response is seen when a constant SRIF concentration is pulsed for variable periods of time. There is no net inhibition of [3H]rGH release by pulses of SRIF.