DEVELOPMENTAL EXPRESSION OF MYELIN PROTEINS BY OLIGODENDROCYTES IN THE CNS OF TROUT
DEVELOPMENTAL EXPRESSION OF MYELIN PROTEINS BY OLIGODENDROCYTES IN THE CNS OF TROUT
复制标题
DOI：
10.1016/0165-3806(90)90255-w
复制
发表时间：
1990-01-01
期刊：
影响因子：
--
通讯作者：
JACQUE, C
中科院分区：
文献类型：
--
作者：
JESERICH, G;MULLER, A;JACQUE, C
利用免疫组织化学技术，细胞质染色的模式和表达的时间顺序的少突胶质细胞的5个主要髓鞘成分在发育中的中枢神经系统的鳟鱼进行了研究。两个髓鞘糖蛋白，IP1和IP2，在胶质细胞的细胞质中呈颗粒状的免疫染色模式，而36K蛋白均匀分布。在髓鞘形成的早期阶段，新鲜分离的细胞的分析揭示了一个恒定的时间序列的表达髓鞘蛋白的少突胶质细胞：糖蛋白IP2是第一个蛋白质出现在胶质细胞的发展过程中与半乳糖苷GalC在第28阶段，随后在第30阶段的36 K，最后在第32阶段的IP1。髓鞘蛋白沉积到新生髓鞘中的时间顺序与少突胶质细胞表达的时间顺序相同。此外，髓鞘碱性蛋白，这是没有检测到的神经胶质细胞，在组织切片上被发现出现与IP2平行。
Using immunohistochemical techniques, the pattern of cytoplasmic staining and the temporal order of expression of 5 major myelin components of oligodendrocytes were studied in the developing central nervous system of trout. The two myelin glycoproteins, IP1 and IP2, in the cytoplasm of glial cells showed a granular pattern of immunostaining, whereas the 36K protein was homogeneously distributed. Analysis of freshly dissociated cells during early stage of myelinogenesis revealed a constant chronological sequence of expression of myelin proteins by the oligodendrocytes: glycoprotein IP2 was the first protein to appear during glial development together with the galactocerebroside GalC at stage 28 followed by the 36K at stage 30 and finally IP1 at stage 32. The deposition of myelin proteins into the nascent myelin sheath occurred in the same chronological order as their expression by oligodendrocytes. Moreover myelin basic protein, which was not detectable in glial cells, on tissue sections was found to appear in parallel with IP2.