Role of anchorage in the expression of tumorigenicity of untransformed mouse cell lines.
Role of anchorage in the expression of tumorigenicity of untransformed mouse cell lines.
复制标题
锚定在未转化小鼠细胞系致瘤性表达中的作用。
DOI：
10.1093/jnci/69.2.415
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
P. Kraemer
中科院分区：
文献类型：
--
作者：
R. S. Wells;E. Campbell;D. Swartzendruber;L. M. Holland;P. Kraemer
培养的小鼠细胞在裸鼠中的致瘤性进行了测试，既有传统的测定（注射细胞悬液）和一个新的测试，涉及植入生长在明胶海绵上的细胞。从不同来源获得的Balb/3 T3细胞的亚系在任一测定中的致瘤潜力不同。一个亚系（A）仅在海绵测定中形成独特的癌前结节;这些结节通常在3-4个月的潜伏期后变得进行性。然而，该亚系的细胞悬浮液也在类似的潜伏期后引起肿瘤，但在肿瘤形成之前没有结节期。Balb/3 T3（M）的另一个亚系在任一测定中都未能形成肿瘤。Balb/3 T3亚系在体外性质上没有差异，例如低饱和密度、不能在甲基纤维素中生长和单层形态。第二种实验方法涉及在不同传代水平下对裸BALB/c小鼠胚胎成纤维细胞的测试。将细胞从原代培养物通过危机传代至异倍体，建立细胞系。在海绵试验中较早证明了致瘤性，此时与恶性行为相关的体外参数puronectin未发生变化。与固体表面肉瘤发生在体内的现象可能的关系进行了讨论。
Cultured mouse cells were tested for tumorigenicity in nude mice with both a conventional assay (injection of cell suspensions) and a new test involving implantation of cells grown on gelatin sponges. Sublines of Balb/3T3 cells, obtained from different sources, varied in their tumorigenic potential with either assay. One subline (A) formed distinctive precancerous nodules only in the sponge assay; these nodules often became progressive after a latent period of 3-4 months. However, suspensions of cells of this subline also caused tumors after a similar latent period, but no nodular phase preceded tumor formation. Another subline of Balb/3T3 (M) has failed to form tumors in either assay. The Balb/3T3 sublines did not differ in vitro properties, such as low saturation density, failure to grow in methylcellulose, and monolayer morphology. A second experimental approach involved tests on nude BALB/c mouse-embryo fibroblasts at various passage levels. The cells were passaged from primary culture, through crisis, to heteroploid, established cell lines. Tumorigenicity was demonstrable earlier in the sponge assay, at which time in vitro parameters putatively associated with malignant behavior were unchanged. Possible relationships with the in vivo phenomenon of solid-surface sarcomagenesis are discussed.