Platinum nanoparticles have an activity similar to mitochondrial NADH:ubiquinone oxidoreductase
Platinum nanoparticles have an activity similar to mitochondrial NADH:ubiquinone oxidoreductase
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DOI：
10.1016/j.colsurfb.2008.06.008
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发表时间：
2008-10-15
影响因子：
5.8
通讯作者：
Miyamoto, Yusei
中科院分区：
文献类型：
--
作者：
Hikosaka, Keisuke;Kim, Juewon;Miyamoto, Yusei
这项研究旨在检查铂纳米颗粒是否具有与线粒体复合物1，NADH：泛醌氧化还原酶相似的活性。铂纳米颗粒通过柠檬酸盐还原H2 PtCl 6制备，并由柠檬酸盐本身和果胶（CP-Pt）保护。在50 μ M CP-Pt存在下，观察到NADH呈时间和剂量依赖性减少，NAD（+）呈时间依赖性增加：这些观察结果是使用分光光度法进行的，其中340和260 nm处的最大吸收光谱分别用于NADH和NAD（+）。在CP-Pt中实现50%的NADH氧化3小时所需的铂浓度约为20 μ M，并且这种NADH氧化不需要氧作为电子受体。我们还使用NAD（+）/NADH定量试剂盒验证了NAD（+）形成。通过将CoQ（1）与CP-Pt在水性缓冲液中孵育，观察到2，3-二甲氧基-5-甲基-6-（3-甲基-2-丁烯基）-1，4-苯醌（CoQ（1））的吸收峰从278 nm移至284 nm。用HPLC进一步分析显示CoQ（1）被CP-Pt还原为CoQ（1）H-2。总的来说，铂纳米颗粒具有NADH：泛醌氧化还原酶样活性。这表明，铂纳米颗粒是一种潜在的药用物质，用于抑制线粒体复合物I的氧化应激疾病。(C)2008 Elsevier B. V.保留所有权利。
This study was designed to examine if platinum nanoparticles have an activity similar to mitochondrial complex 1, NADH:ubiquinone oxidoreductase. Platinum nanoparticles were prepared by a citrate reduction of H2PtCl6 and protected by citrate itself and pectin (CP-Pt). Time- and dose-dependent decreases in NADH and a time-dependent increase in NAD(+) were observed in the presence of 50 mu M CP-Pt: these observations were made using a spectrophotometric method in which the Maximum absorption spectra at 340 and 260 nm were used for NADH and NAD(+), respectively. The required platinum concentration in CP-Pt to achieve a 50% oxidation of NADH for 3 h was approximately 20 mu M, and this NADH oxidation did not require oxygen as an electron acceptor. We also verified NAD(+) formation using an NAD(+)/NADH quantification kit. The absorption peak shift from 278 to 284 nm of 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone (CoQ(1)) was observed by incubating CoQ(1) with CP-Pt in an aqueous buffer. A further analysis with HPLC revealed the reduction of CoQ(1) to CoQ(1) H-2 by CP-Pt. As a whole, platinum nanoparticles have an NADH:ubiquinone oxidoreductase-like activity. This suggests that platinum nanoparticles are a potential medicinal substance for oxidative stress diseases with suppressed mitochondrial complex I. (C) 2008 Elsevier B.V. All rights reserved.