Metabolomic strategies to map functions of metabolic pathways.
Metabolomic strategies to map functions of metabolic pathways.
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绘制代谢途径功能的代谢组学策略。
DOI：
10.1152/ajpendo.00228.2014
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Nomura,DanielK
中科院分区：
文献类型：
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作者：
Mulvihill,MelindaM;Nomura,DanielK
基因组测序工作已经揭示了惊人数量的未注释和未表征的基因，这些基因属于代谢酶类，这可能表明我们目前对正常生理学中的生化途径的了解，更不用说疾病状态了，仍然很不完整。这一认识为后基因组时代的科学家们破译这些酶及其代谢物和代谢网络的生化和（病理）生理作用提出了一个艰巨的挑战。最近的许多研究表明，疾病状态下正常代谢网络的重新布线导致酶、代谢物和代谢途径的独特病理生理功能，这使情况进一步复杂化。本文综述了最近的发现，利用代谢图谱技术发现新的途径和代谢物介导的翻译后修饰和表观遗传改变及其对生理和疾病的影响。
Genome sequencing efforts have revealed a strikingly large number of unannotated and uncharacterized genes that fall into metabolic enzymes classes, likely indicating that our current knowledge of biochemical pathways in normal physiology, let alone in disease states, remains largely incomplete. This realization presents a daunting challenge for post-genomic-era scientists in deciphering the biochemical and (patho)physiological roles of these enzymes and their metabolites and metabolic networks. This is further complicated by many recent studies showing a rewiring of normal metabolic networks in disease states to give rise to unique pathophysiological functions of enzymes, metabolites, and metabolic pathways. This review focuses on recent discoveries made using metabolic mapping technologies to uncover novel pathways and metabolite-mediated posttranslational modifications and epigenetic alterations and their impact on physiology and disease.