A new continuous cell line from the pest insect, Anomala cuprea (Coleoptera; Scarabaeidae): emergence of contractile cells
A new continuous cell line from the pest insect, Anomala cuprea (Coleoptera; Scarabaeidae): emergence of contractile cells
复制标题
DOI：
10.1007/s11626-022-00707-5
复制
发表时间：
2022-07
期刊：
影响因子：
--
通讯作者：
Takafumi N. Sugimoto;Kazuyo Watanabe;G. Akiduki;S. Imanishi;W. Mitsuhashi
中科院分区：
文献类型：
--
作者：
Takafumi N. Sugimoto;Kazuyo Watanabe;G. Akiduki;S. Imanishi;W. Mitsuhashi
昆虫收缩细胞经常出现在细胞培养的早期阶段，但在大多数情况下，它们在建立连续的细胞系之前消失，因此细胞系停止收缩。持续收缩的昆虫细胞系目前仅可从膜翅目和双翅目的一个物种获得。在这里，我们获得了一个新的细胞系，在建立为连续细胞系后很长时间内收缩。在建立连续细胞系之前，该细胞系在昆虫细胞培养的早期阶段收缩了一小段时间，但随后几年内没有再次收缩。在该细胞系进入连续生长期后，它产生自发收缩组织约4个月，但再次停止收缩。这是细胞系在其作为非收缩性连续细胞系建立后收缩的第一个实例。目前尚不清楚收缩细胞在细胞培养的早期阶段停止收缩后是存活还是死亡，我们的研究结果表明，潜在的收缩细胞在停止收缩后存活数年。这条线的细胞有时会产生复杂的收缩结构，如片状组织。只有少数连续的细胞系已从金龟子甲虫。新的连续细胞系来自金龟子甲虫Anomala cuprea的脂肪体的培养，金龟子甲虫是日本农业和林业的害虫。新细胞系的群体倍增时间为2.5 d，因此它在鞘翅目连续细胞系中生长非常迅速。我们的新细胞系将促进对鞘翅目（包括金龟子）生理学和病理学的研究，也可能有助于对无脊椎动物肌肉的研究。
Insect contractile cells frequently appear at an early phase of cell culture, but in most cases, they disappear before a continuous cell line is established, so the cell line ceases to contract. Continuous contractile insect cell lines are currently available from only one species each of Hymenoptera and Diptera. Here, we obtained a new cell line that contracted long after being established as a continuous cell line. The cell line contracted for a short period at an early phase of insect cell culture before a continuous cell line was established, but then did not contract again for several years. After this cell line entered the continuous growth phase, it produced spontaneously contractile tissues for about 4 mo but stopped contracting again. This is the first instance of a cell line that contracted after its establishment as a non-contractile continuous cell line. It is unclear whether the contractile cells survive or die after contraction ceases at an early phase of cell culture, and our results indicate that potential contractile cells survive for years after they stop to contract. The cells of this line sometimes produced complex contractile structures, such as sheet-like tissues. Only a few continuous cell lines have been derived from scarabaeid beetles. The new continuous cell line was derived from the culture of the fat bodies of the scarab beetleAnomala cuprea, which is a pest in the agriculture and forestry of Japan. The population doubling time of the new cell line was 2.5 d and thus it grows very rapidly among coleopteran continuous cell lines. Our new cell line will facilitate research on the physiology and pathology of Coleoptera, including scarab beetles, and may also contribute to research on invertebrate muscles.