Injection of Dip-allatostatin or Dip-allatostatin pseudopeptides into mated female Diploptera punctata inhibits endogenous rates of JH biosynthesis and basal oocyte growth
Injection of Dip-allatostatin or Dip-allatostatin pseudopeptides into mated female Diploptera punctata inhibits endogenous rates of JH biosynthesis and basal oocyte growth
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DOI：
10.1016/s0965-1748(00)00041-2
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发表时间：
2000-08-01
影响因子：
3.8
通讯作者：
Tobe, SS
中科院分区：
文献类型：
--
作者：
Garside, CS;Nachman, RJ;Tobe, SS
对allatostatins（ASTs）的催化活性的研究为设计一系列二酰化allatostatins衍生的假肽模拟类似物提供了理论基础。在体外，Dip-AST和假肽显示出不同程度的对卡替林的抗性，并且都显示出对保幼激素（JH）生物合成的显著抑制。本研究进行，以确定是否有效的Dip-AST和/或其伪肽模拟物对应物引起的“allatostatic”的影响，在体内注射到交配的女性Diploptera punctata。注射水性溶剂或Dip-AST 7（1-7）N-末端片段（不包括AST的活性核心区域）的动物用作对照。体外放化实验表明，注射Dip-AST 5、7或假肽类似物397-2或AST（B）phi 2可显著抑制JH的生物合成（P
Studies on the catabolism of allatostatins (ASTs) provided the rationale for the design of a series of Dip-allatostatin-derived pseudopeptide mimetic analogues. In vitro, the Dip-ASTs and pseudopeptides show varying degrees of resistance to catabolism and all show significant inhibition of juvenile hormone (JH) biosynthesis. This study was undertaken to determine whether potent Dip-ASTs and/or their pseudopeptide mimetic counterparts caused 'allatostatic' effects in vivo following injection into mated female Diploptera punctata. Animals injected with aqueous solvent or Dip-AST 7(1-7) N-terminal fragment, which excludes the active core region of the ASTs, were used as controls. An in vitro radiochemical assay revealed that injection of Dip-AST 5, 7 or pseudopeptide analogues 397-2 or AST(b)phi 2 significantly inhibited the biosynthesis of JH (P