Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells
Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells
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DOI：
10.1007/s10103-011-0968-0
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发表时间：
2012-07-01
影响因子：
2.1
通讯作者：
Sasso Stuani, Maria Bernadete
中科院分区：
文献类型：
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作者：
Bernardes da Silva, Ana Paula R.;Petri, Alice D.;Sasso Stuani, Maria Bernadete
本研究的目的是研究上颌快速扩张（RME）术后低水平激光治疗（LLLT）后中腭缝合线细胞的成骨活性。30只大鼠分为实验I组（RME + LLLT 15只）和实验II组（RME + LLLT 15只）。分别于RME后24小时、48小时和7天处死大鼠，取出大鼠中腭缝线的成骨细胞。将这些细胞培养至17天，然后评估体外成骨参数和基因表达标志物。在RME + LLLT后24和48小时，从中腭缝合处收获的培养细胞在增殖期（3-7天）的细胞倍增时间缩短，这表明在培养中细胞的生长增加。在RME后24、48 h和7 d，中腭缝线外植的细胞在培养第7天和第14天的碱性磷酸酶活性被LLLT提高。在RME后的所有时期，第17天的矿化度均增加。real-time PCR结果显示，与对照细胞相比，RME后LLLT细胞中ALP、Runx2、骨钙素、I型胶原蛋白和骨唾液蛋白mRNA的水平更高。在RME和LLLT后48小时观察到对ALP活性、矿化和骨标志物基因表达的影响更为明显。这些结果表明，RME后应用的LLLT能够增加来自中腭缝合的细胞的增殖和成骨细胞表型的表达。
The aim of this study was to investigate the osteoblastic activity of cells derived from the midpalatal suture upon treatment with low-level laser therapy (LLLT) after rapid maxillary expansion (RME). A total of 30 rats were divided into two groups: experimental I (15 rats with RME without LLLT) and experimental II (15 rats with RME + LLLT). The rats were euthanized at 24 h, 48 h, and 7 days after RME, when the osteoblastic cells derived from the rats' midpalatal suture were explanted. These cells were cultured for periods up to 17 days, and then in vitro osteogenesis parameters and gene expression markers were evaluated. The cellular doubling time in the proliferative stage (3-7 days) was decreased in cultured cells harvested from the midpalatal suture at 24 and 48 h after RME + LLLT, as indicated by the increased growth of the cells in a culture. Alkaline phosphatase activity at days 7 and 14 of the culture was increased by LLLT in cells explanted from the midpalatal suture at 24 and 48 h and 7 days after RME. The mineralization at day 17 was increased by LLLT after RME in all periods. Results from the real-time PCR demonstrated that cells harvested from the LLLT after RME group showed higher levels of ALP, Runx2, osteocalcin, type I collagen, and bone sialoprotein mRNA than control cells. More pronounced effects on ALP activity, mineralization, and gene expression of bone markers were observed at 48 h after RME and LLLT. These results indicate that the LLLT applied after RME is able to increase the proliferation and the expression of an osteoblastic phenotype in cells derived from the midpalatal suture.