Isotope tracer measures of meal fatty acid metabolism: reproducibility and effects of the menstrual cycle
Isotope tracer measures of meal fatty acid metabolism: reproducibility and effects of the menstrual cycle
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DOI：
10.1152/ajpendo.00340.2004
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发表时间：
2005-03-01
影响因子：
5.1
通讯作者：
Jensen, M
中科院分区：
文献类型：
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作者：
Uranga, AP;Levine, J;Jensen, M
膳食脂肪的氧化和脂肪组织摄取可以通过向膳食中添加脂肪酸示踪剂来测量。进行这些研究以测量这些类型的实验的研究间变异性，并评估饮食脂肪酸在月经周期的卵泡期与黄体期是否被不同地处理。健康正常体重的男性（n=12）和女性（n=12）参与了这些研究，这些研究被随机分组，以控制研究顺序、同位素（[H-3]三油酸甘油酯vs.[C-14]三油酸甘油酯）和月经周期。能量消耗（间接量热法），膳食脂肪酸氧化，膳食脂肪酸摄取到上半身和下半身皮下脂肪（活检）后24小时的实验餐进行了测量。大部分膳食脂肪酸储存在上半身皮下脂肪组织中（24+/-2% vs. 16+/-2%，P
Oxidation and adipose tissue uptake of dietary fat can be measured by adding fatty acid tracers to meals. These studies were conducted to measure between-study variability of these types of experiments and assess whether dietary fatty acids are handled differently in the follicular vs. luteal phase of the menstrual cycle. Healthy normal-weight men (n=12) and women (n=12) participated in these studies, which were block randomized to control for study order, isotope ([H-3] triolein vs. [C-14] triolein), and menstrual cycle. Energy expenditure (indirect calorimetry), meal fatty acid oxidation, and meal fatty acid uptake into upper body and lower body subcutaneous fat (biopsies) 24 h after the experimental meal were measured. A greater portion of meal fatty acids was stored in upper body subcutaneous adipose tissue (24+/-2 vs. 16+/-2%, P