Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation
Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation
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DOI：
10.1136/gut.51.4.496
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发表时间：
2002-10-01
期刊：
影响因子：
24.5
通讯作者：
Farrugia, G
中科院分区：
文献类型：
--
作者：
Lyford, GL;He, CL;Farrugia, G
背景：Cajal间质细胞（Interstitial cells of Cajal，ICC）是肠道正常运动所必需的. ICC在整个人类结肠中被发现，并且在慢传输型便秘患者的乙状结肠中减少。目的：本研究的目的是确定ICC在人类结肠内的正常分布，并确定在慢传输型便秘中ICC在整个结肠中是否减少。患者：本研究采用6例慢传输型便秘患者的盲肠、升结肠、横结肠和乙状结肠以及结肠癌切除患者的结肠组织。ICC细胞进行了鉴定与c-Kit的多克隆抗体，系列0.5 μ m切片，通过共聚焦显微镜获得，和三维软件被用来重建整个厚度的结肠固有肌层和粘膜下层。两个网络的ICC被确定，一个在肌间神经丛区域和另一个，不太明确的网络，在粘膜下边界。盲肠、升结肠、横结肠和乙状结肠显示相似的ICC体积。ICC体积显着降低在慢传输型便秘患者在所有的结肠regions.Conclusions：数据表明，ICC分布是相对均匀的整个人类结肠和ICC体积减少是泛结肠在特发性慢传输型便秘。
Background: Interstitial cells of Cajal (ICC) are required for normal intestinal motility. ICC are found throughout the human colon and are decreased in the sigmoid colon of patients with slow transit constipation.Aims: The aims of this study were to determine the normal distribution of ICC within the human colon and to determine if ICC are decreased throughout the colon in slow transit constipation.Patients: The caecum, ascending, transverse, and sigmoid colons from six patients with slow transit constipation and colonic tissue from patients with resected colon cancer were used for this study.Methods: ICC cells were identified with a polyclonal antibody to c-Kit, serial 0.5 mum sections were obtained by confocal microscopy, and three dimensional software was employed to reconstruct the entire thickness of the colonic muscularis propria and submucosa.Results: ICC were located within both the longitudinal and circular muscle layers. Two networks of ICC were identified, one in the myenteric plexus region and another, less defined network, in the submucosal border. Caecum, ascending colon, transverse colon, and sigmoid colon displayed similar ICC volumes. ICC volume was significantly lower in the slow transit constipation patients across all colonic regions.Conclusions: The data suggest that ICC distribution is relatively uniform throughout the human colon and that decreased ICC volume is pan-colonic in idiopathic slow transit constipation.