Human myocardial protein pattern reveals cardiac diseases.
Human myocardial protein pattern reveals cardiac diseases.
复制标题
DOI：
10.1155/2012/342659
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Baykut D
中科院分区：
文献类型：
--
作者：
Bergquist J;Baykut G;Bergquist M;Witt M;Mayer FJ;Baykut D
采用高效液相色谱/傅立叶变换离子回旋共振质谱仪(FT-ICR-MS)对两种不同心力衰竭病因的心肌组织进行蛋白质组学研究。收集10例血流动力学显着的单纯性主动脉瓣病变患者和10例单纯性有症状性冠心病患者的右心耳。正如我们小组早些时候的一项研究(Baykut等人，2006年)所提出的那样，这两种疾病表现出明显不同的模式分布特征。足够有趣的是，分类模式可以用于将未知测试样本正确地分类到正确的类别中。然而，为了充分利用和验证这些发现，确实需要在分子水平上明确地识别不同病因之间的差异。在这项研究中，代表主动脉瓣疾病和冠心病的样本被制备、胰酶消化，并使用FT-ICR MS进行分析，该MS允许选定的分类器团块的碰撞诱导解离(CID)。利用片段图谱，通过数据库检索鉴定蛋白质。为了比较和进一步验证，分类器质量也被碎裂，并使用高效液相/基质辅助激光解吸电离(MALDI)飞行时间/飞行时间(TOF/TOF)质谱仪进行分析。Desmin和Lumcan前体是在主动脉样本中发现的蛋白质的例子，其丰度高于冠状动脉样本。同样，在冠状动脉样本中也发现了丰度较高的腺苷酸激酶同工酶。所描述的方法在寻找血浆或血清中的特定生物标志物用于诊断目的也是可行的。
Proteomic profiles of myocardial tissue in two different etiologies of heart failure were investigated using high performance liquid chromatography (HPLC)/Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Right atrial appendages from 10 patients with hemodynamically significant isolated aortic valve disease and from 10 patients with isolated symptomatic coronary heart disease were collected during elective cardiac surgery. As presented in an earlier study by our group (Baykut et al., 2006), both disease forms showed clearly different pattern distribution characteristics. Interesting enough, the classification patterns could be used for correctly sorting unknown test samples in their correct categories. However, in order to fully exploit and also validate these findings there is a definite need for unambiguous identification of the differences between different etiologies at molecular level. In this study, samples representative for the aortic valve disease and coronary heart disease were prepared, tryptically digested, and analyzed using an FT-ICR MS that allowed collision-induced dissociation (CID) of selected classifier masses. By using the fragment spectra, proteins were identified by database searches. For comparison and further validation, classifier masses were also fragmented and analyzed using HPLC-/Matrix-assisted laser desorption ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry. Desmin and lumican precursor were examples of proteins found in aortic samples at higher abundances than in coronary samples. Similarly, adenylate kinase isoenzyme was found in coronary samples at a higher abundance. The described methodology could also be feasible in search for specific biomarkers in plasma or serum for diagnostic purposes.