Cloning of Tetrahymena genomic sequences whose message abundance is increased during conjugation.
Cloning of Tetrahymena genomic sequences whose message abundance is increased during conjugation.
复制标题
四膜虫基因组序列的克隆，其信息丰度在接合过程中增加。
DOI：
10.1016/s0012-1606(89)80057-0
复制
发表时间：
1989
影响因子：
2.7
通讯作者：
Karrer,KM
中科院分区：
文献类型：
--
作者：
Rogers,MB;Karrer,KM
对嗜热四膜虫（Tetrahymena thermophilicaconjugation）蛋白质调控的分子生物化学研究主要从两个方面进行：新翻译蛋白质的二维凝胶分析和信息丰度增加的基因的分子克隆。二维凝胶分析表明，32个主要是碱性蛋白质的合成刺激接合细胞。这些蛋白质的诱导不能与饥饿的长度或交配类型。对T.嗜热基因组DNA，选择的基础上的差异杂交共轭或控制细胞RNA，进行了研究。两个克隆，这是同源的共轭细胞中检测到的成绩单，显示微和大核DNA之间没有重排。第三个克隆被分成三段。一个片段是同源的序列限于微核。第二个片段与大量经HindHI消化的微核DNA限制性片段杂交，但仅与两个大核DNA片段杂交。第三段，这是互补的一个转录在接合细胞和两个不同的转录在控制细胞，杂交的两个片段在微核DNA和一个片段在大核DNA。
A molecular and biochemical inquiry into protein regulation duringTetrahymena thermophilaconjugation was carried out in two ways: a two-dimensional gel analysis of newly translated proteins and the molecular cloning of genes whose message abundance is increased. The two-dimensional gel analysis indicated that the synthesis of 32 predominantly basic proteins was stimulated in conjugating cells. The induction of these proteins could not be correlated with length of starvation or with mating type. The transcription pattern and molecular organization of three clones ofT. thermophilagenomic DNA, selected on the basis of differential hybridization to conjugating or control cell RNA, were investigated. Two of the clones, which were homologous to transcripts detected in conjugating cells, showed no rearrangements between micro- and macronuclear DNA. A third clone was divided into three segments. One segment was homologous to sequences limited to the micronucleus. A second segment hybridized to a large number of restriction fragments of micronuclear DNA digested withHindHI but to only two fragments of macronuclear DNA. A third segment, which was complementary to one transcript in conjugating cells and to two different transcripts in control cells, hybridized to two fragments in micronuclear DNA and one fragment in macronuclear DNA.
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嗜热四膜虫接合过程中蛋白质合成的阶段特异性变化。
影响因子：
3.7
作者：
P. Suhr
通讯作者：
P. Suhr
DOI：
10.1111/j.1432-1033.1978.tb12413.x
发表时间：
1978-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
ECKERT, WA;KAFFENBERGER, W;FRANKE, WW
通讯作者：
FRANKE, WW
影响因子：
64.5
作者：
GUTTMAN, SD;GLOVER, CVC;GOROVSKY, MA
通讯作者：
GOROVSKY, MA
嗜热四膜虫接合过程中蛋白质合成、膜蛋白和伴刀豆球蛋白 A 结合蛋白的分析。
DOI：
--
发表时间：
1983
期刊：
影响因子：
--
作者：
C. Bell
通讯作者：
C. Bell
影响因子：
--
作者：
Orias, E;Bruns, P J
通讯作者：
Bruns, P J