EMBEDDING IN EPOXY RESINS FOR ULTRATHIN SECTIONING IN ELECTRON MICROSCOPY
EMBEDDING IN EPOXY RESINS FOR ULTRATHIN SECTIONING IN ELECTRON MICROSCOPY
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DOI：
10.3109/10520296009114754
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发表时间：
1960-01-01
期刊：
影响因子：
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通讯作者：
FINKE, EH
中科院分区：
文献类型：
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作者：
RICHARDSON, KC;JARETT, L;FINKE, EH
固定的组织用叔丁醇脱水过夜。第二天，将其在甲苯中澄清，渗透并嵌入不含邻苯二甲酸酯的Araldite树脂-硬化剂-促进剂混合物中，并在60[degree] C下聚合。比以前的技术更快，这种方法得到的块在修剪时不会过度断裂，并提供1 [mu]的软组织切片。用于相差显微镜，以及像甲基丙烯酸酯切片一样容易用玻璃刀切割的切片。美国制造的Araldite。S.A.在英国是不同的。美国人满意的配比为：固化剂DDSA 3.5 ml，铸造树脂6905 5.0 ml，促进剂B 0.12 ml。对于英国产品，这些是：硬化剂964 B，5.0 ml;铸造树脂M，5.0 ml;促进剂964 C，0.25 ml。在Araldite中使用2%琼脂定向小标本是可行的。Mallory的硼砂-亚甲基蓝已被应用于装在载玻片上的薄至0.5 [mu]的Araldite切片的染色。
Fixed tissue is dehydrated with tertiary butyl alcohol overnight. The following day it is cleared in toluene, infiltrated and embedded in Araldite resin-hardener-accelerator mixture without dibutyl phthalate, and polymerized at 60[degree] C. More rapid than previous techniques, this method gives blocks which do not fracture unduly on trimming and provides sections of soft tissues at 1 [mu]. for phase contrast microscopy, as well as ultrathin sections which cut as easily with glass knives as sections of methacrylate. Araldite manufactured in the U. S.A. and in England are different. Satisfactory proportions for the American are: hardener DDSA, 3.5 ml; casting resin 6905, 5.0 ml; accelerator B, 0.12 ml. For the British product, these are: hardener 964 B, 5.0 ml; casting resin M, 5.0 ml; accelerator 964 C, 0.25 ml. The use of 2% agar for orienting small specimens in Araldite is feasible. Mallory''s borax-methylene blue has been applied to the staining of Araldite sections as thin as 0.5 [mu] mounted on glass slides.