Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers
Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers
复制标题
DOI：
10.3791/50815
复制
发表时间：
2013-12-01
影响因子：
1.2
通讯作者：
Granger, Douglas A.
中科院分区：
文献类型：
--
作者：
Hodgson, Nancy A.;Granger, Douglas A.
唾液测量已经出现在生物行为研究中，这种测量容易收集，侵入性最小，并且相对便宜的压力生物标记物。在这篇文章中，我们介绍了两种唾液分析的步骤：唾液皮质醇和唾液α-淀粉酶，这两种唾液分析方法在研究中对虚弱的社区老年人进行。唾液生物科学领域正在迅速发展，本报告的目的是为有兴趣将这些措施整合到衰老研究中的研究人员提供最新进展。介绍了指导家庭照顾者在家中收集唾液的策略，以及对唾液分析物进行实验室分析的策略，这些分析已经证明了可行性、高依从性和高质量的样本。样品采集方案包括：(1)收集材料的一致使用；(2)促进遵守和最大限度减少受试者负担的标准化方法；(3)控制某些混杂物质的程序。我们还提供实验室分析的策略，包括：(1)唾液处理和处理；(2)唾液皮质醇和唾液α-淀粉酶分析程序；以及(3)分析考虑。
Salivary measures have emerged in bio-behavioral research that are easy-to-collect, minimally invasive, and relatively inexpensive biologic markers of stress. This article we present the steps for collection and analysis of two salivary assays in research with frail, community residing older adults-salivary cortisol and salivary alpha amylase. The field of salivary bioscience is rapidly advancing and the purpose of this presentation is to provide an update on the developments for investigators interested in integrating these measures into research on aging. Strategies are presented for instructing family caregivers in collecting saliva in the home, and for conducting laboratory analyses of salivary analytes that have demonstrated feasibility, high compliance, and yield quality specimens. The protocol for sample collection includes: (1) consistent use of collection materials; (2) standardized methods that promote adherence and minimize subject burden; and (3) procedures for controlling certain confounding agents. We also provide strategies for laboratory analyses include: (1) saliva handling and processing; (2) salivary cortisol and salivary alpha amylase assay procedures; and (3) analytic considerations.