Expression of cyclo-oxygenase-2 is correlated with high intratumoral microvessel density and low apoptotic index in human esophageal squamous cell carcinomas
Expression of cyclo-oxygenase-2 is correlated with high intratumoral microvessel density and low apoptotic index in human esophageal squamous cell carcinomas
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DOI：
10.1007/s00428-002-0706-x
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发表时间：
2003-02-01
期刊：
影响因子：
3.5
通讯作者：
Ito, H
中科院分区：
文献类型：
--
作者：
Kase, S;Osaki, M;Ito, H
环氧合酶（考克斯）是花生四烯酸转化为前列腺素的关键酶。考克斯-2在多种恶性肿瘤中均有表达。本研究分析了食管癌组织中考克斯-2表达与血管生成和细胞凋亡的关系。本研究应用免疫组化和TUNEL法检测了6株食管癌细胞系和100例食管鳞癌组织中考克斯-2的表达，比较了肿瘤内微血管密度（IMVD）和凋亡指数（AI）。考克斯-2在所有检测的细胞系中均呈弱阳性表达。考克斯-2免疫反应主要位于癌细胞的胞浆中。51例考克斯-2强表达病例的平均IMVD显著高于49例弱表达病例，AI显著低于49例弱表达病例。IMVD与AI呈负相关。考克斯-2在有淋巴结转移的肿瘤中的表达高于无淋巴结转移的肿瘤。这些数据表明，考克斯-2表达与肿瘤内微血管增加和肿瘤细胞凋亡抑制有关。因此，考克斯-2可能在食管鳞癌血管生成和细胞凋亡调控中发挥重要作用。
Cyclo-oxygenase (COX) is a key enzyme in the conversion of arachidonic acid to prostanoids. COX-2 expression has been found in many malignancies. This study analyzed the correlation between COX-2 expression and angiogenesis or apoptosis in human esophageal carcinomas. The study examined the expression of COX-2 in six esophageal carcinoma cell lines and in 100 esophageal squamous cell carcinomas, comparing intratumoral microvessel density (IMVD) and apoptotic index (AI) by immunohistochemistry and TUNEL methods. COX-2 was variably expressed in all the cell lines examined. COX-2 immunoreactivity was observed mainly in the cytoplasm of carcinoma cells. Significantly higher mean IMVD and lower AI were noted in the 51 strong COX-2 expressing cases than in the 49 weak cases. IMVD and AI were negatively correlated. COX-2 expression was higher in the tumors with lymphatic invasion than in the others. These data indicate that COX-2 expression is associated with increased intratumoral microvessels and suppression of tumor cell apoptosis. Thus COX-2 might play an important role in the angiogenesis and regulation of apoptosis in esophageal squamous cell carcinomas.